Effects of dihydromyricetin on apoptosis of lung adenocarcinoma A549 cells
Author(s): SUN Da-yong, ZUO Yan-zhen, LIANG Hong-hu, SONG Ji, MA Chun-hu, SUN Hao-yang, Department of Tumor Radiation and Chemotherapy Center, Chengde Central Hospital, Department of Pharmacology, Chengde Medical University, Gink Fok Kang Medication Group corporation limited
Pages: 359-362
Year: 2015 Issue: 3
Journal: Acta Anatomica Sinica
Keyword: Dihydromyricetin; A549 cell; Lung cancer; Western blotting; Flow cytometry; Human;
Abstract: Objective To study the effect of dihydromyricetin on apoptosis of hunman non-small cell lung cancer A549 cells,and to explore new drugs for lung cancer. Methods A549 cells were treated with various concentrations of dihydromyricetin for 48 hours. MTT assay was used to determined the proliferation ability of A549 cells,flow cytometry was used to detect the influence of dihydromyricetin on the cell apoptosis of A549 cells,Western blotting analysis was used to evaluate the levels of Bax,Bcl-2 expressions. Results MTT assay results showed a significant inhibition in A549 cells with a dose-dependent manner,when compared with control cells,the inhibitory concentration for 50%( IC50) was64. 45 mg / L at 48 hours; Flow cytometry showed that dihydromyricetin induced apoptosis of A549 cells in a dose-dependent manner as well. Further analysis by Western blotting showed that the expression of Bax was increased,while the expression of Bcl-2 was reduced when A549 cells was incubated for 48 hours with dihydromyricetin. Conclusion Dihydromyricetin plays a role of anti-tumor through inhibiting A549 cells proliferation and inducing apoptosis.
Pages: 359-362
Year: 2015 Issue: 3
Journal: Acta Anatomica Sinica
Keyword: Dihydromyricetin; A549 cell; Lung cancer; Western blotting; Flow cytometry; Human;
Abstract: Objective To study the effect of dihydromyricetin on apoptosis of hunman non-small cell lung cancer A549 cells,and to explore new drugs for lung cancer. Methods A549 cells were treated with various concentrations of dihydromyricetin for 48 hours. MTT assay was used to determined the proliferation ability of A549 cells,flow cytometry was used to detect the influence of dihydromyricetin on the cell apoptosis of A549 cells,Western blotting analysis was used to evaluate the levels of Bax,Bcl-2 expressions. Results MTT assay results showed a significant inhibition in A549 cells with a dose-dependent manner,when compared with control cells,the inhibitory concentration for 50%( IC50) was64. 45 mg / L at 48 hours; Flow cytometry showed that dihydromyricetin induced apoptosis of A549 cells in a dose-dependent manner as well. Further analysis by Western blotting showed that the expression of Bax was increased,while the expression of Bcl-2 was reduced when A549 cells was incubated for 48 hours with dihydromyricetin. Conclusion Dihydromyricetin plays a role of anti-tumor through inhibiting A549 cells proliferation and inducing apoptosis.
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