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Effects of prenatal nitrite exposure on hippocampus of mouse pups
Pages: 289-296
Year: Issue:  3
Journal: Acta Anatomica Sinica

Keyword:  Nitrite exposureHippocampal inguryOxidative stressComet assayWestern blottingMouse;
Abstract: Objective To investigate the effects on hippocampus after prenatal nitrite exposure. Methods C57 BL /6J mice were used to establish the models of prenatal nitrite exposure with control group( physiological saline),low dose nitrite exposure group( 60 mg / kg) and high dose nitrite exposure group( 120 mg / kg). The pups of different groups at P0,P7,P14 and P30 were gathered for immunofluorescence,comet assay and Western blotting analysis to detect the hippocampal injury. Results In both nitrite exposure group and control group,the number of proliferative neuron in dentate gyrus gradually decreased with the growing age. At postnatal day 0( P0),P7,P14 and P30,after prenatal nitrite exposure,5-bromo-2-deoxy Uridine( Brd U) positive cells decreased with dose dependency( P < 0. 05,n = 96). In order to identify if the inhibtion was selective,we observed Brd U positive cells in subventricular zone( SVZ) at P0 mouse. After nitrite exposure,Brd U positive cells in SVZ also decreased with dose dependency( P < 0. 05). The inflammatory injury and apoptosis cells in hilus increased with dose dependency after nitrite exposure( P < 0. 05). The tail of hippocampus cells in nitrite exposure groups was longer than that in control groups with dose dependency( P < 0. 01). The expressions of Caspase-8 and nuclear factor κB( NF-κB) protein in nitrite exposure groups in hippocampus were much higher than that in control groups( P < 0. 05). Conclusion Prenatal nitrite exposure can induce various hippocampus injuries,such as neuroproliferative inhibition,oxidative stress and neuroapoptosis.
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