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da shu gan da bu qie chu hou gan zai sheng de yan jiu ying yong liu shi xi bao ji ce ding gan xi bao zeng zhi zhou qi dong tai
Author(s): 
Pages: 374
Year: Issue:  4
Journal: Acta Anatomica Sinica

Keyword:  肝细胞流式细胞计肝再生静脉灌注肝大肝组织切片雄性成年大鼠分离制备细胞悬液细胞涂片;
Abstract: <正> 雄性成年大鼠36只,3只为正常对照;33只做肝大部切除。切除分11组,每组3例。分别于术后12~120小时内的不同间隔时间取材。动物经门静脉灌注去血,分离制备肝细胞悬液,荧光(EB)染色,用国产流式细胞计(FCM)测定肝细胞DNA含量,以鸡红细胞为内插标准,每例动物测5~6×104个肝细胞。同时制作肝组织切片和肝细胞涂片,分别计数肝细胞分裂指数(MI)和双核肝细胞数。正常大鼠肝细胞DNA含量有2 n、4 n、8 n组型,以4 n肝细胞占优势(76%)。肝大部切除后20小时,部分4 n肝细胞快速分裂,2 n肝细胞比例增加,双核肝细胞减少。术后24小时出现4 n肝细胞S期(平均为11.5%)和G2期(8 n)高峰。8 n肝细胞为
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