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THE STUDIES OF HISTOCHEMISTRY AND ELECTRON-MICROSCOPIC CYTOCHEMISTRY ON SDH AND LDH IN THE MYOCARDIUM OF EXPERIMENTAL ANOXIC MICE
Author(s): 
Pages: 210-215+239
Year: Issue:  2
Journal: Acta Anatomica Sinica

Keyword:  小鼠心肌LDHSDH琥珀酸脱氢酶乳酸脱氢酶线粒体膜肌原纤维水浸液酶活性线粒体酶;
Abstract: With the methods of light microscopic histochemistry and electron-microscopic cytochemistry, we studied the changes of lactate dehydrogenase (LDH) and succinic dehydrogenase (SDH) in the anoxic myocardium of the mice; to which normal saline (INSAM group) or 10% aqueous extract of Ginsengradix rubra (IGRAM group) was injected before the anoxic experiment. The results were as follows:Ⅰ. The Light Microscopic Study:1. In the myocardial sections of the INSAM group, the reaction of SDH of myofibrillae are variegated and the fibrillae vary in thickness. Granular mitochondria are clearly stained and arranged in alignment in the intermyofibrillar spaces. The intercalated discs fail to be stained and their spaces widen. In the IGRAM group, the reactions of SDH are similar to that of the normal control. The enzyme reaction product in the myofibrillae appear as homogeneous diffuse blue precipitate of formazan, the cross striations of myofibrillae are clear and strings of mitochondria are visible.2. The reactions of LDH in the INSAM group reveals that myofibrillar staining is also lost in a length of myofibrilla, strings of mitochondria are clearly stained. The reaction in the IGRAM group is similar to that of the normal control. In the myofibrillae there is a homogenous continuous violet precipitate of formazan.Ⅱ. The Electron-Microscopic Study:1. The reaction of SDH in the INSAM group shows that the enzyme activity in the mitochondrial inner membrane and the cristae is stronger than that of the normal control. The increased activity is represented by the increase of electronic density or darkening and thickening of the membranes. However, the activity in the IGRAM group is simillar to that of the normal control but weaker in reaction.2. The reaction of LDH in the INSAM group reveals that the enzyme activity exhibited by membrane darkening is obviously stronger than that of the normal control but in the IGRAM group it shows clearly a weaker reaction.The results mentioned above suggest that Ginsengradix rubra might alleviate the metabolic disturbance caused by myocardial anoxia.
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