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TOPOGRAPHY OF THE NIGRONEOSTRIATUM PROJECTION IN THE RAT——A FLUORESCENCE HISTOCHEMICAL STUDY COMBINED WITH HRP TECHNIQUE
Author(s): 
Pages: 253-259+340
Year: Issue:  3
Journal: Acta Anatomica Sinica

Keyword:  黑质纹状体荧光组织化学致密部尾壳核标记细胞网状部腹侧内侧部局部定位;
Abstract: 0.02~0.3μl of 33% HRP (Sigma Ⅵ) solution in sterile water were injected with a stereotaxic instrument into various sites of the caudate-putamen in 40 rats. The animals were perfused transaortically with 3% paraformatdehyde and 1% glutaradehyde in 0.1 M phosphate buffer solution (pH7.4). Frozen sections were dried in a container with P2O5 for 2~4 hours and examined for fluorescence in the nucleus nigra. After which, the sections were processed for HRP reaction according to Mesulam’s method. A number of cells of substantia nigra were found to show both fluorescence and HRP-positive product, thus confirming the dopaminergic nature of the nigroneostiatum projection.Furthermore, the nigroneostriatum projection was found to be topographically organized, the ventro-medial part of compact zone of the substantia nigra projecting to the dorso-medial caudate-putamen, the dorso-medial part of compact zone to the ventral caudate-putamen, the ventro-middle of compact zone to 1be dorso-lateral and central caudate-putamen, the caudo-lateral part to the central caudate-putamen, the middle part of reticular zone to the lateral and central caudate-putamen, the lateral zone of substantia nigra to the caudal caudate-putamen. Some overlap existed.Strionigral fibers were also topographically organized, which terminated predominantly about the cells of reticular zone. These may form a feedback loop concerning with the modulation of the activity of the dopaminergic neurons in the substantia nigra.
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