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EFFECT OF DIETHYINITROSAMINE ON THE LIVER OF RAT--ESTABLISHMENT OF THE MODEL OF HEPATOMA AND CARCINOCENESIS
Author(s): 
Pages: 205-211+233-234
Year: Issue:  2
Journal: Acta Anatomica Sinica

Keyword:  肝癌模型二乙基亚硝胺肝细胞癌变增生结节肝细胞肝癌癌变过程总剂量实验动物模型建立过程致癌作用;
Abstract: Hepatocellular carcinoma.was induced in all the male albino rats with an average body weight of 145±25.8gm by feeding diethylnitrosanaine(DEN)96.1±1.61 to 185 ±0 mg,during 90±1.73 to 195±0 days. Many hyperplastic and carcinogenic nodules instead of normal lobular structure were observed in the affected liver.The maximum diameter of the carcingenic nodule is 3.5 cm. The tumour tissues can be divided into three types:A,B,and M.Type A is com- posed of large-sized acidophilic hepatomic cells which are arranged in the form of hepatocarcinoma muraliosum duplex or triplex.Type B is composed of small-sized baso- philic hepatomic cells which are arranged in the form of hepatocarcinoma muraliosum multiplex.Type M is composed of mixed acidophilic and basophilic hepatomic cells. When 98.1±1.61 mg of DEN was fed in a duration of 90±1.73 days only Type A hepatomic tissue was induced,while 123±2.35 mg in a duration of 123±3.81 days was fed all the three types of hepatomic tissue were observed in liver and also meta- stases of the hepatonaic tissue. Large-sized hepatomic cells with multiple nuclei were found in all the three types of hepatomic tissue.The metastatic hepatomic tissue belongs to Type A or B. The hepatomic cells are PAS-negative in contrast with the normal strongly PAS- positive hepatic cells;in hyperplasnc nodules;and more DNA and RNA inhepatomic nodules thanin normal cells.The basophilic substance in the hepatomic cells appears in the form of concentric lamellae,reticulum,rough or dust-like particles.The precarcinogenic or carcinogenic area in liver is characterized by the decrease of glycogen,increase of baso- philia and nuclear DNA,and the change of hepatocellular muraliosum from simplex to multiplex. From the above observation the author advances a hypothesis that the hepatomic tissue arises from the burst of cell division of the hepatic cells in the hyperplastic nodules.
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