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China/Asia On Demand
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Pages: 212-217+236
Year: Issue:  2
Journal: Acta Anatomica Sinica

Keyword:  乳酸脱氢酶活性血液有形成分胆碱酯酶乳酸钠血涂片脏象内环境特异性活性物质乙酞胆碱;
Abstract: The cholinesterase(ChE)and lactate dehydrogenase(LDH)activities in blood smears of 37 patients were observed before and 20 minutes after electroacupuncture.Hegu and Zusanli were mainly chosen as the acupuncture points.Karnovsky method was used to demonstrate ChE,activity,and tetrazolium-formazan reaction for LDH activity. Before acupuncture,all formed elements of blood showed both ChE and LDH acti- vities.In red blood cells ChE and LDH activities were localized at the cell membrane, while in white blood cells they were found throughout the cytoplasm as colored granu- les.The granulocytes showed greater ChE and lesser LDH activities than the lymph- ocytes.The platelets also showed greater LDH and ChE activities,especially the former. On the whole,platelets and leucocytes,as compared with erythrocytes,showed greater activities for ChE and LDH. Under microscopic observation,according to the amount and color of granules,the degrees of GhE and LDH activities for each kind of blood formed elements before and after acupuncture were recorded as different markes,such as +、++、+++ and so on.The degrees of GhE or LDH activity in various formed elements of blood were compared by means of statistical tests.After acupuncture,both GhE and LDH activities of all blood formed elements were increased(p<0.01).The ChE activity in platelets was increased more markedly than in erythrocytes(p<0.05).Both platelets and leukocytes showed a more marked increase of LDH activity than erythrocytes(p<0.01). Regarding the effects of acupuncture,it was shown that there was a significant increase of ChE activity of all blood formed elements in the excellent and good efficacy groups(p<0.05),while in the poor efficacy group,the alteration of ChE activity was insignificant(p<0.05).In the excellent efficacy group,there was an increase of LDH activity of all blood formed elements(p<0.05).In the good efficacy group,only leu- cocytes and platelets showed an increase of LDH activity,while in the poor efficacy group, platelets alone showed an increase of LDH activity(p<0.05). In most cases,LDH activity of the erythrocytes in the excellent efficacy group enhanced considerably.However,in several cases with higher level of LDH activity before acupuncture,the decrease or no alteration of the LDH activity was found.It was sug- gested therefore that the electroacupuncture may play a role in the regulation of LDH activity in erythrocytes.On the whole,ChE、LDH activities in all formed elements of blood enhanced markedly after acupuncture,and the physiological significance was discussed in this paper.
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