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HISTOLOGICAL AND HISTOCHEMICAL STUDY OF THE EFFECT OF THE ADMINISTRATION OF LARGE DOSES OF CORTISONE ACETATE ON THE RAT LIVER
Author(s): 
Pages: 374-392
Year: Issue:  4
Journal: Acta Anatomica Sinica

Keyword:  肝糖原动物肝动物体重糖原含量臀部肌肉注射实验动物核甙酸解剖学报肝细胞激素合成;
Abstract: Forty-nine adult male albino rats, weighing between 140--210 gms were selectedfor study. Among them, 9 served as controls and the rest were injected daily with 25 mgof cortisone or hydrocortisone into the gluteal muscles and they were sacrificed after 1,2, 3, and 6 injections and 4, 5, and 9 days after the 6th injection. Liver blocks fromboth experimental and control groups were taken immediately after death and were pro-cessed with routine histological and histochemical techniques. After the administration of cortisone, the eosinophilic granules, protein-bound tyro-sine, histidine and tryptophan, sulfhydryl and disulfide groups, and ribonucleic acid con-spicuously decreased in amount in liver cells. Hence these cells were filled with clearvacuoles of various sizes in the cytoplasm and assumed the name of "balloon cells." Cortisone caused an immediate rise of liver glycogen and phosphorylase activity. The activity of acid and alkaline phosphatases increased in the liver tissues in thecarly stages of treatment and the change of the latter enzyme was more apparen.Later, it was reduced and did not recover to the normal level even on the 9th day afterthe last injection. The activity of adenosine triphosphatase and 5-nucleotidase, elevatedat the beginning of the treatment, returned to normal afterwards. The activity of nonspecific phosphatase in the liver tissues of all the animals of theexperimental group was kept at a lower level than in the normal controls. Nonspecificesterase reacted in the same but more exaggerated manner. Cytochrome oxidase activity in the liver cells apparently decreased after the adminis-tration of cortisone, but it gradually recovered after the injection was suspended. The influence of cortisone and hydrocortisone upon glycogen, ribonucleic acid, andvarious enzymes was the same, but they showed different actions on proteins and pro-tein-bound sulfhydyl and disulfide groups. Maximum depletion of the latter substanceswas attained after a single injection of cortisone, it required 6 injections of hydrocor-tisone to get a similar effect apparently the action of the former compound is more im-mediate and more pronounced than that of the latter.
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