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MEKK3 siRNA recombinant adenovirus vector transduction induces apoptosis of human liver cancer cell line HCC-9204 with TRAIL
Author(s): 
Pages: 720-726
Year: Issue:  12
Journal: World Clinical Drugs

Keyword:  liver cancer cell linemitogen-activated protein kinase/extracellular signal regulated kinase-kinase 3TRAILsiRNAadenovirus vector;
Abstract: Objective To investigate the promoting effect of silencing MEKK3 gene on TRAIL-induced apoptosis of liver cancer HCC-9204 cells, so as to search for a novel treatment for liver cancer. Methods MEKK3 siRNA recombinant adenovirus vector was constructed and transduced into liver cancer HCC-9204 cells. RT-PCR and Western blotting was performed to analyze the suppression of MEKK3 in the transduced cells. Flow cytometry was used to measure TRAIL- induced apoptosis. Results DNA sequencing and RT-PCR confirmed that the MEKK3 siRNA expression adenovirus vectors were successfully constructed. RT-PCR and Western blotting showed that MEKK3 stably and effectively silenced HCC-9204 cells were established. The significant increasing of TRAIL(500 g/L)-induced apoptosis was observed in MEKK3 silenced HCC-9204 cells by flow cytometry analysis. Conclusion The MEKK3 stably and effectively silenced liver cancer HCC-9204 cells were successfully established, the correlation between MEKK3 expression and TRAIL- induced apoptosis was demonstrated. The MEKK3 silenced HCC-9204 cells provide a tool for further investigation of the mechanism.
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