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dao shu guang pu tong shi kuai su ce ding he cheng zuo zhong se an suan he lao an suan
Author(s): 
Pages: 379-380
Year: Issue:  6
Journal: World Clinical Drugs

Keyword:  合成肽合成肤紫外光谱分析导数光谱Hewlett吸收度分析纯衍生化反应光谱法二阶导数;
Abstract: <正> 本文报道一种同时快速测定合成肽中色氨酸和酪氨酸含量的二阶导数光谱法,本法不需要水解或衍生化反应程序。试剂光谱分析用的L-色氨酸和L-酪氨酸为分析纯。紫外光谱分析采用Hewlett-Packard8452A型分光光度计二阶导数光谱法记录吸收度值。将合成肽溶解在0.1NNaOH溶液中,制成约200nmol/ml浓度的溶液,分别以0.1NNaOH配制相同浓度的色氨酸和酪氨酸标准溶液为对照进行扫描。
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