Author(s): ZHANG Hao-yue, XU Su-jun, TANG Xiao-xian, NIU Ji-jun, GUO Xiang-jie, GAO Cai-rong
Pages: 188-190
Year: 2015 Issue:  3
Journal: Journal of Forensic Medicine
Keyword:  forensic pathology; anaphylaxis; non-ionic contrast m edia; basophil activation test; C D 63; m ast cell carboxypeptidase A 3; SC 5b-9;
Abstract: Objective To investigate the diagnostic significance of basophil activation test (B A T) in ana-phylaxis to non-ionic contrast media through testing the content of CD 63, m ast cell-carboxypeptidase A 3 (M C-CPA 3), and term inal com plem ent com plex SC5b-9 of the individuals by testing their levels in the norm al im m une group and the anaphylaxis groups to β-lactam drugs and non-ionic contrast media. Methods The CD 63 expression of basophilic granulocyte in blood w as detected by flow cytom etry. The levels of M C-CPA 3 in blood serum and SC5b-9 in blood plasm a w ere detected by ELISA . Results The CD 63 expression of basophilic granulocyte in blood, the levels of M C-CPA 3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs w ere significantly higher than that in norm al im m une group (P<0.05). Conclusion There is activation of basophilic granulocytes, m ast cells and com plem ent system in anaphylaxis to non-ionic contrast media. B A Tcan be used to diagnose the anaphylaxis to non-ionic contrast media.