Author(s): LI HuiYu, ZHAO Peng, GE XiZhen, TIAN PingFang, Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, College of Biochemical Engineering, Beijing Union University
Pages: 87-92
Year: 2014 Issue:  2
Journal: Journal of Beijing University of Chemical Technology(Natural Science Edition)
Keyword:  phosphoenolpyruvate carboxylase(PEPC); CO2fixation; Synechocystis sp.PCC 6803; Acinetobacter baumannii; Escherichia coli;
Abstract: We have engineered three recombinant E.coli overexpressing distinct phosphoenolpyruvate carboxylase(PEPC) from Synechocystis sp.PCC 6803,Acinetobacter baumannii and Escherichia coli.SDS-PAGE analysis showed high expression of PEPC except in the case of the PEPC from A.baumannii.Consistent with this result,unlike the E.coli harboring PEPC from A.baumannii,the other two recombinants overexpressing PEPC entered a log phase earlier than a control that carried a blank vector.More interestingly,all above three recombinant E.coli strains presented nearly equal biomass to wild-type strain after 24 h fermentation.Taken together,if excluding the metabolic burden arising from plasmid replication,PEPC could be a general driving force facilitating cell proliferation due to the reallocation of metabolic flux.