Author(s): GAO Xu-dong, SHI Yong-qing, CHEN Shi-en, HAO Bao-cheng, LIANG Jian-ping, College of Life Sciences and Engineering, Northwest University for Nationalities, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Gansu Key Laboratory of New Veterinary Drug Project, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, College of Chemical Engineering, Northwest University for Nationalities
Pages: 409-412
Year: 2015 Issue:  3
Journal: International Journal of Pharmaceutical Research
Keyword:  HPLC; dual wavelength; Zhizi Jinhua Pills; quantitative analysis;
Abstract: Objective To develop an HPLC method for simultaneous determination of jasminoidin,baicalin,berberine hydrochloride and emodin in Zhizi Jinhua(ZZJH)Pills. Methods The analysis was performed on a Zorbax Eclipse XDB-C18(4.6 mm×150mm,5 μm) with gradient elution by using the mobile phase of acetonitrile-0.2% phosphoric acid solution. The column temperature was maintained at room temperature and the flow rate was 1.0 ml/min. The detection wavelengths were set at 240 nm and 275 nm,respectively. Results The calibration curves were linear within the range of 0.056~0.3360 μg(r=0.9998) for gardenoside,0.208~0.624 μg(r=0.9990)for baicalin,0.116~0.696 μg(r=0.9994)for berberine hydrochloride,and 0.040~0.240 μg(r=0.9993) for emodin,respectively. The recoveries of gardenoside,baicalin,berberine hydrochloride and emodin were 91.18 %(RSD =1.60%)、91.55%(RSD =0.75%)、91.19%(RSD =1.08%) and 92.29%(RSD =1.98%),respectively. Conclusion The method is convenient,fast,sensitive and reproducible,with good precision,specificity and accuracy. So it can be used for simultaneous determination of jasminoidin,baicalin,berberine hydrochloride and emodin in ZZJH Pills.