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cDNA cloning, sequence analysis and tissue expression of Apaf-3 gene in goats
Pages: 285-290
Year: Issue:  3
Journal: Journal of Southwest University for Nationalities(Natrual Science Edition)

Keyword:  山羊Apaf-3基因定量PCR;
Abstract: 对单胎藏山羊和多胎金堂黑山羊Apaf-3基因cDNA克隆、序列分析及组织表达特性研究.采集5只多胎金堂黑山羊和5只单胎藏山羊在发情期的卵巢、垂体等组织样,进行Apaf-3基因的cDNA克隆、序列分析,并采用定量PCR技术对其mRNA进行组织表达量研究.结果,克隆出山羊Apaf-3基因长度为1731bp编码区全长为1245bp,编码414个氨基酸.Apaf-3基因在两种山羊中序列相同,且在5种组织中的表达均无差异.同源性分析表明凋亡基因却妒-3在动物的进化中保守性低,与山羊多羔性状的相关性需要进一步研究.
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