Genome wide expression analysis of the effect of Socheongryong Tang in asthma model of mice
Author(s): Chi-Yeon Lim, Hyung-Woo Kim, Bu-Yeo Kim, Su-In Cho, College of Medicine, Dongguk University, School of Korean Medicine, Pusan National University, Herbal Medicine Research Division, Korea Institute of Oriental Medicine
Pages: 168-174
Year: 2015 Issue: 2
Journal: Journal of Traditional Chinese Medicine
Keyword: Asthma; Transcription factors; Microarray analysis; Genome wide expression; Socheongryong Tang(Xiaoqinglong Tang);
Abstract: OBJECTIVE:To investigate the molecular effect of Socheongryong Tang(SCRT,Xiaoqinglong Tang in Chinese) on whole genome level in asthma mouse model by microarray technology.METHODS:Asthma was induced by intranasal instillation of ovalbumin in mouse.After administration of SCRT on asthma-induced mouse,the expression of genes in lung tissue was measured using whole genome microarray.The functional implication of differentially expressed genes was performed using ontological analysis and the similarity of promoter structure of genes was also analyzed.RESULTS:Treatment of SCRT restored expression level of many up- or down-regulated genes in asthma model,and this recovery rate means SCRT could regulate a set of genes having specific TFBS binding sites.CONCLUSION:In this study,we identified a set of genes subjected to similar regulation by SCRT in asthma model in mice.
Pages: 168-174
Year: 2015 Issue: 2
Journal: Journal of Traditional Chinese Medicine
Keyword: Asthma; Transcription factors; Microarray analysis; Genome wide expression; Socheongryong Tang(Xiaoqinglong Tang);
Abstract: OBJECTIVE:To investigate the molecular effect of Socheongryong Tang(SCRT,Xiaoqinglong Tang in Chinese) on whole genome level in asthma mouse model by microarray technology.METHODS:Asthma was induced by intranasal instillation of ovalbumin in mouse.After administration of SCRT on asthma-induced mouse,the expression of genes in lung tissue was measured using whole genome microarray.The functional implication of differentially expressed genes was performed using ontological analysis and the similarity of promoter structure of genes was also analyzed.RESULTS:Treatment of SCRT restored expression level of many up- or down-regulated genes in asthma model,and this recovery rate means SCRT could regulate a set of genes having specific TFBS binding sites.CONCLUSION:In this study,we identified a set of genes subjected to similar regulation by SCRT in asthma model in mice.
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