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Expression and roles of regulatory B cells and T cells in patients with chronic hepatitis B
Pages: 48-51
Year: Issue:  1
Journal: Journal of Zhenjiang Medical College

Keyword:  regulatory B cellschronic hepatitis Bregulatory T cellsIL-10;
Abstract: Objective: To investigate the expression and roles of IL-10+CD19+regulatory B cells( Breg) and CD4+CD25highregulatory T cell( Treg) in patients with chronic hepatitis B. Methods: A total of 28 patients with acute hepatitis B( AHB),31 patients with chronic hepatitis B( CHB) and 25 normal subjects( HCs group) were enrolled in this study. Peripheral blood mononuclear cells( PBMC) were isolated and stimulated with CpG ODN 2006 and PMA. The expression of IL-10+CD19+Breg and CD4+CD25high Treg was analyzed with flow cytometry. The level of IL-10 in culture supernatant was analyzed with ELISA. CD4+CD25+T cells were removed with Immunomagnetic beads to analyze the interactions between Breg and Treg. Results: After stimulation with CpG ODN 2006 and PMA,compared with the AHB group and the HCs group,percentages of Breg and Treg in the CHB group increased significantly( Breg: P = 0. 003,0.001; Treg: P < 0. 001,< 0. 005),and no difference was found between the AHB group and the HCs group( Breg: P =0. 796; Treg: P =0. 227). Spearman correlation analysis found that in the CHB group,Breg and Treg was positively correlated( r = 0. 50,P = 0. 004),but in the AHB group and the HCs group,there was no such correlation( r =- 0. 15,P = 0. 462; r = 0. 09,P = 0. 669). IL-10 expression in the CHB group was higher than that in the AHB group and the HCs group( Z =- 6. 596,- 6. 373,P < 0. 001),and the difference between the AHB group and the HCs group was not statistically significant( Z =- 0. 952,P = 0. 341). Spearman correlation analysis showed that in the CHB group,IL-10 and Breg were positively correlated( r =3. 303,P =0. 005). Before and after removal of CD4+CD25+T cells,in the CHB group,the difference between Breg and IL-10 expression was not statistically significant. Conclusion: The expression of Breg and Treg increased in patients with chronic hepatitis B,Breg might play a regulatory role by IL-10 secretion.
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