The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Bookmark and Share
Differential expression of seven mitochondrial enzymes in young and old skeletal muscles
Pages: 22-25+30
Year: Issue:  1
Journal: Journal of Zhenjiang Medical College

Keyword:  agingmusclemitochondrionenzymes;
Abstract: Objective: To study the relationship between muscular function and ageing by comparing the morphologic and enzymatic expressions of muscular mitochondrion in different age groups. Methods: Specimens of neck muscles were collected from 7 young patients and 33 old patients who underwent neck surgery. The morphological changes of mitochondrial were demonstrated by electronic microscope,whereas the expressions of NADH dehydrogenase( ubiquinone) 1 alpha subcomplex 1( NDUFA1),succinate dehydrogenase complex( SDHB),cytochrome b( Cytb),cytochrome c oxidase( COX),vacuolar-type H+-ATPase( V-ATPase H),cytosolic malate dehydrogenase( MDH) and hydroxyacyl-coenzyme A dehydrogenase( HADHSC) were detected by immunohistochemical staining and western blotting. Results: Compared with the young patients,the mitochondria in elder patients became larger,and had more accumulating vacuoles, cristae abnormalities and intra-mitochondrial paracrystalline inclusions; meanwhile,the expressions of NDUFA1,SDHB,Cytb,COX,V-ATPase H decreased significantly in elder patients than young patients( both P <0. 05). However,there was no difference in expression of MDH and HADHSC between young patients and old patients. These results were consistent with western blotting. Conclusion: The morphologicand enzymatic function of muscular mitochondria degenerated along with ageing,which might play an important role in ageing.
Related Articles
No related articles found