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Application of AtheNA Multi-Lyte system in the detection of antinuclear antibodies
Pages: 44-47
Year: Issue:  1
Journal: Journal of Zhenjiang Medical College

Keyword:  antinuclear antibodyline immunoassayAtheNA Multi-Lyte system;
Abstract: Objective: To access the detection property of AtheNA system in antinuclear antibodies and anti-dsDNA antibodies. Methods: Ninety and three serum samples were tested utilizing the AtheNA system and LIA method: 16 samples from patients with systemic lupus erythematosus( SLE),57 samples from other known autoimmune diseases and 20 healthy donors. The antinuclear autoantibodies to nine different analytes( SSA,SSB,Sm,RNP,Jo-1,Scl-70,dsDNA,Centromere B and Histone) were tested. Meanwhile, the dsDNA antibodies were tested using line immunoassay( LIA),IIF,ELISA and the AtheNA system. Results: There were no significant difference in the antinuclear antibodies detected in three subjects. Antinuclear autoantibodies screening by AtheNA system revealed high concordance with the LIA in samples obtained from 16 samples from patients with SLE,20 healthy donors,57 samples from other known autoimmune diseases samples,respectively. The test results of 4 methods showed that the sensitivity and specificity of AtheNA system was better in anti-dsDNA antibody test( 68. 8%,83. 1%) than other three methods. Conclusion: The AtheNA system is a sensitive and automatic method for simultaneous quantitative detection of antinuclear antibodies and anti-dsDNA antibodies profiles.
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