Effects of phylloquinone on hepatic oxidative stress and inflammatory factors in ovariectomized rats
Author(s): PAN Chun-fang, XU San-rong, School of Clinical Medicine, Jiangsu University, Department of Gynaecology and Obstetrics, Jintan Traditional Chinese Medical Hospital, Department of General Surgery, the Affiliated Hospital of Jiangsu University
Pages: 31-35
Year: 2014 Issue: 1
Journal: Journal of Zhenjiang Medical College
Keyword: phylloquinone; ovariectomy; liver; oxidative stress; IL-6; TNF-α;
Abstract: Objective: To observe the effects of phylloquinone supplementation on hepatic oxidative stress and inflammation in ovariectomized rats and to propose a new way to prevent the hepatic impairment associated with menopause. Methods: Thirty female SD rats of six months age were randomly divided into 3 groups,sham group( Sham,n = 10),ovariectomy group( OVX,n = 10),phylloquinone supplementation after ovariectomy group( OVX + VK,n = 10). One week after operation,phylloquinone or glycerol which served as delivery agent was administrated through gastric lavage to OVK + VK group or Sham and OVX group respectively for 90 days. After that,the livers were harvested to determine malonaldehyde( MDA), superoxide dismutase( SOD) and glutathione( GSH) levels. Hematoxylin eosin( HE) and reactive oxygen species( ROS) fluorescent staining were performed on hepatic frozen sections. The expressions of IL-6 and TNF-α in the livers were evaluated by Western blotting. Results: The hepatic MDA levels in the OVX + VK group were significantly lower than those in the OVX group and higher than those in the Sham group( both P <0. 05). The hepatic SOD and GSH levels in the OVX + VK group were significantly lower than those in the OVX group,but were not significantly different from those in the Sham group. HE stainingdemonstrated that the small vacuoles in the OVX + VK group were obviously fewer than those in the OVX group while the livers in Sham group contained no vacuole. The ROS fluorescence in the hepatic sections in the OVX + VK group was weaker than that in the OVX group. The results of Western blotting demonstrated that the levels of IL-6 and TNF-α in the OVX + VK group located between the other two groups while those in the OVX group were higher than those in the Sham group. Conclusion: The liver of ovariectomized rats was under higher oxidative stress concomitant with higher levels of inflammatory factors as compared with that of normal rats. These changes can be alleviated by phylloquinone supplementation to some extents.
Pages: 31-35
Year: 2014 Issue: 1
Journal: Journal of Zhenjiang Medical College
Keyword: phylloquinone; ovariectomy; liver; oxidative stress; IL-6; TNF-α;
Abstract: Objective: To observe the effects of phylloquinone supplementation on hepatic oxidative stress and inflammation in ovariectomized rats and to propose a new way to prevent the hepatic impairment associated with menopause. Methods: Thirty female SD rats of six months age were randomly divided into 3 groups,sham group( Sham,n = 10),ovariectomy group( OVX,n = 10),phylloquinone supplementation after ovariectomy group( OVX + VK,n = 10). One week after operation,phylloquinone or glycerol which served as delivery agent was administrated through gastric lavage to OVK + VK group or Sham and OVX group respectively for 90 days. After that,the livers were harvested to determine malonaldehyde( MDA), superoxide dismutase( SOD) and glutathione( GSH) levels. Hematoxylin eosin( HE) and reactive oxygen species( ROS) fluorescent staining were performed on hepatic frozen sections. The expressions of IL-6 and TNF-α in the livers were evaluated by Western blotting. Results: The hepatic MDA levels in the OVX + VK group were significantly lower than those in the OVX group and higher than those in the Sham group( both P <0. 05). The hepatic SOD and GSH levels in the OVX + VK group were significantly lower than those in the OVX group,but were not significantly different from those in the Sham group. HE stainingdemonstrated that the small vacuoles in the OVX + VK group were obviously fewer than those in the OVX group while the livers in Sham group contained no vacuole. The ROS fluorescence in the hepatic sections in the OVX + VK group was weaker than that in the OVX group. The results of Western blotting demonstrated that the levels of IL-6 and TNF-α in the OVX + VK group located between the other two groups while those in the OVX group were higher than those in the Sham group. Conclusion: The liver of ovariectomized rats was under higher oxidative stress concomitant with higher levels of inflammatory factors as compared with that of normal rats. These changes can be alleviated by phylloquinone supplementation to some extents.
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