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Effect of Hyperbaric Oxygen in Combination with 32P-colloid on Implanted Tumor Growth and Serum VEGF Expression in Rats
Author(s): Li Li, Yang Zhijie, Department of Nuclear Medicine, The First Affiliated Hospital of Harbin Medical University
Pages: 49-
52
Year: 2013
Issue:
10
Journal: Journal of Medical Research
Keyword: Hyperbaric oxygen; 32P-colloid; Implanted tumor; VEGF;
Abstract: Objective To investigate the effect of 32P- colloid therapy immediately after hyperbaric oxygen treatment on implanted tumor growth and serum VEGF expression in rats.Methods Immediate Walker- 256 cancerous ascetic injection was used to make a rat tumor- bearing model,then the 1w later 80 rats were randomly divived into control group(A group),hyper- baric oxygen group(B group),32P- colloid group(C group),cooper- ration of hyperbaric oxygen and32P- colloid group(D group),with 20 in each group.Ratsin A and C group by the direct injection of tumors in vivo were respectively given the same volume of saline and32P- colloid.Ratsin B group were treated with hyperbaric oxygen therapy for 14 days after the injection of saline.Ratsin D group were given hyperbaric oxygen therapy for 14 days after the injectin of32P- colloid.Ratsin Every group were measured tumor volume on 7th,12th and 20th day to calculate its growth rate.The vascular endothelial growth factor(VEGF) was performed by immunohistochemistry method.Results The tumor growth rates of the 12th and 20th day in A,B,C and D groups were 232.12% ± 67.52% and 878.64% ± 425.36%,208.23% ± 78.78% and 845.02% ± 632.67%,145.65% ± 55.37% and 502.06% ± 312.96%,104.18% ± 43.75% and 113.86% ± 42.38% respeltirely.The rate of D group was significantly less than those of A,B and C groups(P < 0.05).The rate of C group was obviously less than those of A and B groups(P < 0.05).There was no distinguished difference between the rates of A and B groups(P > 0.05).The VEGF positive expression rates in A,B,C and D groups were 90.0%(18 /20),80.0%(16 /20),30.0%(6 /20),10.0%(2 /20).There was no significant difference between A and B groups(P > 0.05).The positive expression of A and B groups was significantly higher than that of C and D groups(P < 0.05).There was distinguished difference between C and D groups(P < 0.05).Conclusion The tumor growth was inhibited significantly after the treatment of hyperbaric oxygen in combination with32P- colloid.The mechanism may being the expression and the angiogenesis of blood vessel being inhibited,then the proliferation of cancer cells being inhibited.
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