Establishment and Optimization of Multi-gene Transformation System of Populus
Author(s): Zhu Yongxing, Zhang Yan, Wu Hao, Wu Dongpo, Research Center of Agro-biotechnology, Ningxia Academy of Agricultural Sciences, Ningxia Agricultural Comprehensive Development Office
Pages: 43-46
Year: 2015 Issue: 1
Journal: Chinese Agricultural Science Bulletin
Keyword: ‘Ningyang No.1’; Agrobacterium; multi-gene; transformation;
Abstract: In order to establish and optimize the multi- gene transformation system of Populus,‘Ningyang No.1’was used as acceptor material, and multi-gene transformation system was established. SOS1, SOS2 and SOS3 genes related to salt resistance were co-transformed. The results showed that the most suitable medium of adventitious bud was MS+ 6-BA 1.0 mg/L+ NAA 0.5 mg/L, the most suitable screening concentration of herbicide for the leaf piece was 0.8 mg/L, and the most suitable agrobacterium suspension concentration was OD600=0.4. The 14 lines of PCR positive were obtained using this system.
Pages: 43-46
Year: 2015 Issue: 1
Journal: Chinese Agricultural Science Bulletin
Keyword: ‘Ningyang No.1’; Agrobacterium; multi-gene; transformation;
Abstract: In order to establish and optimize the multi- gene transformation system of Populus,‘Ningyang No.1’was used as acceptor material, and multi-gene transformation system was established. SOS1, SOS2 and SOS3 genes related to salt resistance were co-transformed. The results showed that the most suitable medium of adventitious bud was MS+ 6-BA 1.0 mg/L+ NAA 0.5 mg/L, the most suitable screening concentration of herbicide for the leaf piece was 0.8 mg/L, and the most suitable agrobacterium suspension concentration was OD600=0.4. The 14 lines of PCR positive were obtained using this system.
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