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Cloning and expression analysis of a UBX domain-containing protein gene LmU BX2 from the migratory locust,Locusta migratoria manilensis( Orthoptera: Locustidae)
Pages: 361-366
Year: Issue:  4
Journal: Acta Entomologica Sinica

Keyword:  Locusta migratoria manilensisUBX domain-containing proteinLmU BX2gene cloningexpression pattern;
Abstract: 【Aim】Ubiquitin regulatory X( UBX) domain-containing proteins function as cofactors of p97 / CDC48 which is involved in multiple ubiquitin-related processes including protein degradation through the ubiquitin-proteasome system and homotypic membrane fusion. Our study aims to clone the UBX domain-containing protein gene from Locusta migratoria manilensis,and to analyze its expression patterns in different tissues and at different developmental stages,so as to provide the foundation for further function research. 【Methods】A transcriptome database for L. migratoria manilensis was mined through bioinformatic analysis. Gene expression levels were analyzed in different tissues of L. migratoria manilensis adults and at different developmental stages using real-time PCR. 【Results】A UBX domaincontaining protein gene was identified,and named LmU BX2. The open reading frame( ORF) encodes a protein of 339 amino acids with a predicted molecular weight of 37. 8 k Da and a theoretical p I of 6. 03.BLAST analysis showed that it shares 37%- 64% amino acid sequence identities to other UBX domaincontaining proteins. LmU BX2 possesses a conserved N-terminal UBA domain and a C-terminal UBX domain. Sequence comparison and phylogenetic analysis revealed that LmU BX2 is a member of the SAKS1 subfamily. Gene expression analysis results showed that LmU BX2 was expressed during the whole life cycle,and highly expressed in ovary and testis. 【Conclusion】These findings suggest that LmU BX2 might be involved in multiple physiological processes of L. migratoria manilensis. Especially,LmU BX2 might be related to the reproduction of L. migratoria manilensis,although further research needs to be performed to confirm this correlation.
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