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Comparison of Nephrotoxicity Induced By Mongolian Meng-Gen-Wu-Su(Mercury) Processed Products, Mongolian Meng-Gen-Wu-Su(Mercury)-18-Composition Pill and Mercuric Sulfide, Mercuric Chloride and Mercurous Chloride
Pages: 698-706
Year: Issue:  3
Journal: World Science and Technology-Modernization of Traditional Chinese Medicine

Keyword:  Meng-Gen-Wu-Su processed productsMeng-Gen-Wu-Su-18-composition pillmercuric compoundsacute toxicity;
Abstract: The renal toxicity of rats after a single dose of Meng-Gen-Wu-Su(mercury) processed products, MengGen-Wu-Su(mercury)-18-composition pill, mercuric sulfide, mercuric chloride, and mercurous chloride was studied. Fifty-four male Wistar rats were randomly divided into nine groups according to body weights(6 rats in each group): normal control group, low and high dose groups(0.033, 0.33 g·kg-1·d-1) of Meng-Gen-Wu-Su(mercury) processed products, low and high dose groups(0.29, 2.9 g·kg-1·d-1) of Meng-Gen-Wu-Su(mercury)-18-composition pill, simplified prescription of Meng-Gen-Wu-Su(mercury)-18-composition pill group(0.26 g·kg-1·d-1), mercuric sulfide group(17.39 mg·kg-1·d-1), mercuric chloride group(4.06 mg·kg-1·d-1) and mercurous chloride group(35.3 mg·kg-1·d-1). After acclimation for one week, once oral administration was given to each group of rats. After 24 h, function and morphological changes of liver and kidney were detected. Mercury accumulation in kidney was determined by inductively coupled plasma optical emission spectroscopy(ICP-OES) and inductively coupled plasma source mass spectrometer(ICP-MS). Apoptosis of renal cell was determined by terminal-deoxynucleoitidyl transferase mediated Nick End Labeling(TUNEL). Renal type Ⅲ collagen protein’s expression was determined by immunohistochemical(HIC) method and expression changes of MT-1, MT-2 m RNA in kidney were also determined by real-time fluorescence quantitative PCR(real-time-PCR). There was no significant difference of ALT, AST in serum between normal control group and other groups(P>0.05). CREA and UREA in mercurous chloride group were apparently higher than normal control group and low dose group of Meng-Gen-Wu-Su processed products(P<0.01). Hepatic and renal pathologic examination results showed that liver cell of low dose groups of Meng-Gen-Wu-Su processed products and Meng-Gen-Wu-Su-18-composition pill swelled to a low degree and glomerular disease was not obvious. In high-dose groups of Meng-Gen-Wu-Su processed products, MengGen-Wu-Su-18-composition pill and mercuric sulfide group, liver and kidney appeared some pathological changes and such changes were more significant in mercuric chloride and mercurous chloride groups. Compared with normal control group and low dose group of Meng-Gen-Wu-Su processed products, the mercury kidney volume in mercuric chloride and mercurous chloride groups increased significantly(P<0.01). The apoptosis rate of renal cell and expression of type Ⅲ collagen protein increased significantly in the groups of mercuric sulfide, mercuric chloride and mercurous chloride(P<0.01). MT-1and MT-2 m RNA gene expression rised significantly in the groups of mercuric chloride and mercurous chloride(P<0.05 or P<0.01). In summary, the rats renal toxicity after a single dose of Meng-Gen-Wu-Su(Mercury) processed products or Mongolian Meng-Gen-Wu-Su(Mercury)-18-composition pill were both far less than that of mercuric chloride or mercurous chloride.
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