Effects of Chinese medicine Jinlida on hepatic oxidative stress and JNK, p38MAPK signaling pathway in rats fed with high-fat diet
Author(s): LIU Yi-xuan, ZANG Sha-sha, SONG Guang-yao, WANG Chao, REN Lu-ping, DUAN Li-yuan, Graduate School, Hebei Medical University, Department of Endocrinology, Hebei General Hospital, Department of Clinical Medical Research Center, Hebei General Hospital
Pages: 2156-2159
Year: 2015 Issue: 6
Journal: China Journal of Traditional Chinese Medicine and Pharmacy
Keyword: Jinlida; Oxidative stress; Insulin resistant; Liver;
Abstract: Objective: To investigate the effects of Chinese medicine Jinlida on hepatic oxidative stress and JNK, p38 MAPK signal pathway in rats fed with high-fat diet. Methods: Male SD rats aged six weeks were randomly divided into normal control group and high-fat diet group, and rats in normal control group were fed with normal diet, and rats in high-fat diet group were fed with high-fat diet. Six weeks later, rats in high-fat diet group were randomly divided into high-fat diet group, low, middle, and high dosage of Jinlida groups and metformin group. After eight weeks of treatment, hyperinsulin-euglycemic clamp were performed to evaluate the whole-body insulin sensitivity of rats. The rats were sacrificed and the liver samples were collected for measuring hepatic oxidative stress parameters. ROS content in hepatocyte was measured by DHE staining. The total protein and phosphorylation protein expression of JNK, p38 MAPK were determined by using Western blot method. Results: Compared with high-fat diet group, Jinlida could increase glucose infusion rate(P<0.05), decrease the level of hepatic oxidative stress, reduce the formation of LPO(P<0.05) and ROS, increase GSH level, and enhance the activity of SOD, GSH-Px, CAT(P<0.05), and the expression of p-JNK/JNK and p-p38MAPK/MAPK of rats in Jinlida group was significantly decreased(P<0.05). Conclusion: Jinlida could improve insulin sensitivity and reduce hepatic oxidative stress of rats fed with high-fat diet, which might relate to the inhibition effect of Jinllida on JNK and p38 MAPK signal pathway.
Pages: 2156-2159
Year: 2015 Issue: 6
Journal: China Journal of Traditional Chinese Medicine and Pharmacy
Keyword: Jinlida; Oxidative stress; Insulin resistant; Liver;
Abstract: Objective: To investigate the effects of Chinese medicine Jinlida on hepatic oxidative stress and JNK, p38 MAPK signal pathway in rats fed with high-fat diet. Methods: Male SD rats aged six weeks were randomly divided into normal control group and high-fat diet group, and rats in normal control group were fed with normal diet, and rats in high-fat diet group were fed with high-fat diet. Six weeks later, rats in high-fat diet group were randomly divided into high-fat diet group, low, middle, and high dosage of Jinlida groups and metformin group. After eight weeks of treatment, hyperinsulin-euglycemic clamp were performed to evaluate the whole-body insulin sensitivity of rats. The rats were sacrificed and the liver samples were collected for measuring hepatic oxidative stress parameters. ROS content in hepatocyte was measured by DHE staining. The total protein and phosphorylation protein expression of JNK, p38 MAPK were determined by using Western blot method. Results: Compared with high-fat diet group, Jinlida could increase glucose infusion rate(P<0.05), decrease the level of hepatic oxidative stress, reduce the formation of LPO(P<0.05) and ROS, increase GSH level, and enhance the activity of SOD, GSH-Px, CAT(P<0.05), and the expression of p-JNK/JNK and p-p38MAPK/MAPK of rats in Jinlida group was significantly decreased(P<0.05). Conclusion: Jinlida could improve insulin sensitivity and reduce hepatic oxidative stress of rats fed with high-fat diet, which might relate to the inhibition effect of Jinllida on JNK and p38 MAPK signal pathway.
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