Eexpression and Significance of Ptch and Smo in the Tongue Cancinoma Tca8113 Cell lines
Author(s): CHENG Zhi-fen, CUI Yan, XUAN Yan-hua, Department of Stomatology, the First Affiliated Hospital of Nanyang Medical College
Pages: 433-436
Year: 2015 Issue: 5
Journal: Journal of Oral Science Research
Keyword: Hedgehog signaling pathway Tca8113cells Cyclopamine Ptch Smo;
Abstract: Objective:To investigate the expression and significance of the hedgehog signaling pathway factors Ptch and Smo in the tongue cancinoma Tca8113 cell lines.Methods:Aftter treatment of Tca8113 cells with hedgehog signaling pathway antagonist Cyclopamine,the proliferation-inhibition rate was detected by MTT assay.And mRNA and protein levels of Ptch and Smo were analyzed by RT-PCR and Western blot,respectively.Results:Cyclopamine inhibited Tca8113 cells.In the different times treatment of Tca8113 cells with Cyclopamine,the expression of Ptch and Smo mRNA in the Cyclopamine groups was lower than the control group,and the protein expression was the same with mRNA.In the 24 htreatment of Tca8113 cells with Cyclopamine,the expression of Smo mRNA in the Cyclopamine groups was in dose-dependent manner,and in 72 hthe expression of Smo protein in the Cyclopamine groups was in dose-dependent manner.Conclusion:Cyclopamin specifically inhibited the Tca8113 cells migration and inhibited the expression of Smo mRNA and protein.The Hh signaling might be involved in the carcinogenesis and progression of tongue cancer.
Pages: 433-436
Year: 2015 Issue: 5
Journal: Journal of Oral Science Research
Keyword: Hedgehog signaling pathway Tca8113cells Cyclopamine Ptch Smo;
Abstract: Objective:To investigate the expression and significance of the hedgehog signaling pathway factors Ptch and Smo in the tongue cancinoma Tca8113 cell lines.Methods:Aftter treatment of Tca8113 cells with hedgehog signaling pathway antagonist Cyclopamine,the proliferation-inhibition rate was detected by MTT assay.And mRNA and protein levels of Ptch and Smo were analyzed by RT-PCR and Western blot,respectively.Results:Cyclopamine inhibited Tca8113 cells.In the different times treatment of Tca8113 cells with Cyclopamine,the expression of Ptch and Smo mRNA in the Cyclopamine groups was lower than the control group,and the protein expression was the same with mRNA.In the 24 htreatment of Tca8113 cells with Cyclopamine,the expression of Smo mRNA in the Cyclopamine groups was in dose-dependent manner,and in 72 hthe expression of Smo protein in the Cyclopamine groups was in dose-dependent manner.Conclusion:Cyclopamin specifically inhibited the Tca8113 cells migration and inhibited the expression of Smo mRNA and protein.The Hh signaling might be involved in the carcinogenesis and progression of tongue cancer.
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