Triggering receptor expressed on myeloid cells 2 in synovial tissue of rheumatoid arthritis rats
Author(s): Ye Pei, Li Jian-hua, Xu Jin-huang, Huang Sheng-hui, Liu Gui-wang, Zhang Wei-qiong, Zheng Pei-zhong, Huang Jian-rong, Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Department of Physiology, Gugangzhou Medical University, Department of Orthopedics, Zengcheng People’s Hospital
Pages: 2807-2813
Year: 2015 Issue: 18
Journal: Journal of Clinical Rehabilitative Tissue Engineering Research
Keyword: Tissue Engineering; Arthritis; Rheumatoid; Arthritis; Experimental; Synovial Membrane; Cytokines;
Abstract: BACKGROUND: Triggering receptor expressed on myeloid cells 2(TREM-2) is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis still remains unclear. OBJECTIVE: To explore the TREM-2 expression in the synovial tissue of collagen type II-induced arthritis rats. METHODS: The collagen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamically observed. The mR NA levels of TREM-2, tumor necrosis factor-α,interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively. RESULTS AND CONCLUSION: At day 13 after immunization, the paws of model rats appeared red and swelling, the arthritis index scores were increased(P < 0.01). At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of collagen-induced arthritis rats were proliferated and were infiltrated by inflammatory cells, cartilage was destroyed. Compared with the control group, the expression of TREM-2 m RNA and protein, the m RNA levels of tumor necrosis factor-α and interleukin-1β in synovial tissue of the model rats were significantly increased(P < 0.05 or P < 0.01), while interleukin-10 m RNA expression was significantly decreased(P < 0.05). Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of collagen-induced arthritis.
Pages: 2807-2813
Year: 2015 Issue: 18
Journal: Journal of Clinical Rehabilitative Tissue Engineering Research
Keyword: Tissue Engineering; Arthritis; Rheumatoid; Arthritis; Experimental; Synovial Membrane; Cytokines;
Abstract: BACKGROUND: Triggering receptor expressed on myeloid cells 2(TREM-2) is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis still remains unclear. OBJECTIVE: To explore the TREM-2 expression in the synovial tissue of collagen type II-induced arthritis rats. METHODS: The collagen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamically observed. The mR NA levels of TREM-2, tumor necrosis factor-α,interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively. RESULTS AND CONCLUSION: At day 13 after immunization, the paws of model rats appeared red and swelling, the arthritis index scores were increased(P < 0.01). At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of collagen-induced arthritis rats were proliferated and were infiltrated by inflammatory cells, cartilage was destroyed. Compared with the control group, the expression of TREM-2 m RNA and protein, the m RNA levels of tumor necrosis factor-α and interleukin-1β in synovial tissue of the model rats were significantly increased(P < 0.05 or P < 0.01), while interleukin-10 m RNA expression was significantly decreased(P < 0.05). Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of collagen-induced arthritis.
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