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miRNA Master Regulators Drosha and Dicer is Associated with Endometrial Cancer
Author(s): 
Pages: 514-518
Year: Issue:  3
Journal: Journal of Clinical Research

Keyword:  Ribonuclease ⅢRNASmall InterferingMicroRNAsEndometrial Neoplasms/PA;
Abstract: [Objective]To explored the possible roles of Dicer and Drosha in endometrial cancer .[Meth‐ods] Quantitative reverse transcription‐polymerase chain reaction (RT‐PCR and Western blot were used to de‐tect the mRNA and protein levels for Dicer and Drosha in endometrial cancer and normal endometrial cells .Af‐ter transfections of Dicer or Drosha small hairpin RNA (shRNA) in normal endometrial cells ,cell proliferation and apoptosis were detected by methyl thiazolyl tetrazolium (MTT) and flow cytometry .Furthermore ,the levels of Bcl‐2 and Bad were detected by Western blot .[Results] The mRNA and protein levels for Dicer and Drosha in endometrial cancer decreased significantly than normal endometrium .After transfection of Dicer/Drosha shRNA ,increased cell proliferation and decreased cell apoptosis were detected in normal endometrial cells .Furthermore ,Bcl‐2 increased while Bad deceased .In comparison ,a scramble siRNA transfection did not affect the protein levels .[Conclusion] Low expressions of Dicer and Drosha are significantly correlated with endometrial cancer .And shDicer or shDrosha can affect cell proliferation and apoptos so that manipulating Dic‐er and Drosha may yield novel treatment strategies for endometrial cancer .
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