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HPLC Determination of Chlorogenic Acid in Jiangzhining Tblets
Pages: 64-66
Year: Issue:  5
Journal: Journal of Liaoning College of Traditional Chinese Medicine

Keyword:  Jiangzhining Tbletschlorogenic acidHPLCcontent determination;
Abstract: Objective:To determine the content of chlorogenic acid in Jiangzhining Tblets. Methods:HPLC was used to determine the content of Chlorogenic Acid. A Inertsilx C18(250 mm×4. 6 mm,5 μm)column was used. The mobile phase was acetonitrile-0.4% phosphoric acid(9 ∶ 91)and the detection wavelength was 327 nm;The injection volume was 10 μL and column temperature was kept at 30℃;the flow rate was 1 m L·min-1. Results:The calibration curve of Chlorogenic Acid was in good linearity over the range of 0.013 16~0.263 2 μg(r=0.999 9). The average recovery was 98.26% with RSD of 1.12%.Conclusion:The method is simple,accurate and reliable,which can be used for the quality control of Jiangzhining Tblets.
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