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RP-HPLC with DAD Switch Determination of Hydroxysafflor Yellow A and Ferulic acid in Dieda Huoxue Powders
Author(s): 
Pages: 62-64
Year: Issue:  5
Journal: Journal of Liaoning College of Traditional Chinese Medicine

Keyword:  Dieda Huoxue Powdershydroxysafflor yellow Aferulic acidcontent determination;
Abstract: Objective:To establish an RP HPLC method for simultaneously determining two components in Dieda Huoxue Powders,including hydroxysafflor yellow A and ferulic acid. Methods:The determination was performed on a Waters Symmetry C18(250 mm×4.6 mm,5 μm),the mobile phase consisted of methanol-0.2% formic acid at a flow rate of 1.0 m L/min with 30 ℃. DAD was used as the detector. The detection wavelengths were 403 nm(0~11 min)and 324 nm(11~20 min). Results:The method has a good linearity in the ranges of 0.248~2.48 μg for hydroxysafflor yellow A and 0.08~0.80 μg for ferulic acid,respectively(r ≥ 0.999 9). The average recoveries of the two compoments were more than 95.0%. Conclusion:This method with strong specificity is simple,accurate and can be used for quality control of Dieda Huoxue Powders.
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