Effect of major royal jelly proteins (MRJPs) on proliferation activity of Chang's liver cell line and their mechanism of action
Author(s): Yu Zhangying, Chen Di, Wang Yiran, Shen Lirong
Pages: 7-14
Year: 2015 Issue: 1
Journal: Journal of Zhejiang University(Agriculture & Life Sciences)
Keyword: major royal jelly proteins (MRJPs); Chang's liver cell line; fetal bovine serum (FBS); proliferation; substitution; cell cycle;
Abstract: 将王浆主蛋白(major royal jelly proteins,MRJPs)添加到细胞培养基中,用MTT法比较纯MRJPs与胎牛血清(fetal bovine serum,FBS)、不同MRJPs/FBS比例对张氏肝细胞增殖的影响,用流式细胞仪检测MRJPs各处理对细胞周期的影响.结果表明:仅添加MRJPs不能促进细胞增殖;完全培养基(10％ FBS)中MRJPs的最佳添加量为0.5 mg/mL; MRJPs(5 mg/mL)/FBS的添加比例为60/40时混合培养基对细胞的促增殖效果与完全培养基相比差异无统计学意义(P＞0.05).流式细胞仪检测结果显示,MRJPs与FBS配合使用可促使细胞S期及G0/G1期比例增大;推测MRJPs的作用可能与促进DNA合成、前期RNA和核糖体合成有关.
Pages: 7-14
Year: 2015 Issue: 1
Journal: Journal of Zhejiang University(Agriculture & Life Sciences)
Keyword: major royal jelly proteins (MRJPs); Chang's liver cell line; fetal bovine serum (FBS); proliferation; substitution; cell cycle;
Abstract: 将王浆主蛋白(major royal jelly proteins,MRJPs)添加到细胞培养基中,用MTT法比较纯MRJPs与胎牛血清(fetal bovine serum,FBS)、不同MRJPs/FBS比例对张氏肝细胞增殖的影响,用流式细胞仪检测MRJPs各处理对细胞周期的影响.结果表明:仅添加MRJPs不能促进细胞增殖;完全培养基(10％ FBS)中MRJPs的最佳添加量为0.5 mg/mL; MRJPs(5 mg/mL)/FBS的添加比例为60/40时混合培养基对细胞的促增殖效果与完全培养基相比差异无统计学意义(P＞0.05).流式细胞仪检测结果显示,MRJPs与FBS配合使用可促使细胞S期及G0/G1期比例增大;推测MRJPs的作用可能与促进DNA合成、前期RNA和核糖体合成有关.
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