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wei xiao rna-222 zai ji xing que xue xing nao zu zhong xue qing zhong de biao da ji qi dui gai diao dan bai de diao kong zuo yong
Author(s): 
Pages: 765-767
Year: Issue:  5
Journal: The Journal of Practical Medicine

Keyword:  卒中微小RNA-222钙调蛋白;
Abstract: 目的:检测微小RNA-222(miR-222)在急性缺血性脑卒中(AIS)患者血清中的表达,并探讨其对钙调蛋白(CaM)的调控机制。方法:采用实时荧光定量PCR法测定106例AIS患者(AIS组)及98例正常对照者(对照组)血清中miR-222表达情况;用生物信息学数据库查询分析miR-222对钙调蛋白编码基因(CALM1)的靶向调控关系;脂质体转染法将miR-222模拟物、抑制物及对照物分别转染至人脐静脉内皮细胞(HUVEC)中并进行细胞培养,采用逆转录PCR和Western blot分别检测干预后HUVECs钙调蛋白mRNA和蛋白的表达水平。结果:与对照组相比,AIS组miR-222表达明显下调(P <0.01);CALM1存在miR-222潜在靶向结合位点;HUVECs 转染miR-222模拟物后CALM1/CaM表达水平较对照组显著下降(P <0.05),而转染miR-222抑制物后表达水平较对照组显著增高(P <0.05)。结论:AIS患者血清miR-222表达明显下降,其可能通过上调CaM的表达从而加重AIS的损害作用。
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