Author(s): HAN Li-hong, YANG Wen-jie, GAO Li-jun, SU Yan, QIN Wen-bin
Pages: 62-63
Year: 2015 Issue:  5
Journal: Guide of Chinese Medicine
Keyword:  Starch-agarose electrophoresis; Fetal hemoglobin; HbF; HbA2; Cross interaction;
Abstract: ObjectiveWe have demonstrate that adult hemoglobin HbA1 and HbA2 can cross interact each other by starch-agarose mixed gel electrophoresis, but the fetal hemoglobin HbF was not studied at that time. At present, we use this method to study whether the interaction between HbF and HbA2 occur.MethodsSeparating red blood cells from the umbilical cord, then preparation pure hemoglobin F from the starch-agarose electrophoresis gel electrophoresis, and let hemoglobin F dissolve blood through adult red blood cells was observed after dissolving blood hemoglobin A2 through the deformation zone.ResultsThe dissolution of erythrocyte hemoglobin F in the blood through the adult hemoglobin A2, which is deformed as compared with the free hemoglobin F, hemoglobin in the blood solution into the F swimming speed slower.ConclusionThe results showed that the cross interaction was occurred as well between HbF and HbA2.