Application of improved PAS staining in acute liver injury
Author(s): Zhang Feng, Tao Yanyan, Chen Gaofeng
Pages: 178-181
Year: 2015 Issue: 1
Journal: Chinese Journal of Bioprocess Engineering
Keyword: Hepatic glycogen; Acute hepatic injury; Periodic acid-schiff (PAS); Improvement;
Abstract: Objective:To study in situ staining of glycogen by modified Periodic acid-schiff (PAS)staining on evaluating acute hepatic injury induced by carbon tetrachloride (CCl4)in rats.Methods:Rats were randomly divided into 2 groups:normal group and model group.Except normal rats,all the other rats in model group were injected subcutaneously 1 00% CCl4 3mL/kg once, then 50% CCl4 2mL/kg two times a week for 4 times.All the rats were sacrificed on the 1 5th day.Liver body ratio of rats was calculated;The serum parameters of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bilirubin (TBil), albumin (Alb)were assayed.Hepatic inflammation was observed with HE staining.Hepatic glycogen was stained by routine PAS staining and improved PAS staining.Results:Compared with the normal group,the activities of ALT and AST and TBil content in-creased significantly (P<0. 05 ).Alb content was significantly decreased compared with the normal group (P<0. 05 ).HE stai-ning showed that serious structural damage,hepatic steatosis and ballooning degeneration were seen in model group.Routine PAS staining showed that PAS positive staining area accounted for 32.38% ±5.50% in normal group.Compared with the normal group,PAS positive staining area decreased significantly (8.60%±3.34 %,P<0. 01 ).Improved PAS staining showed that PAS positive staining area accounted for 75 .50%±9.02 % in normal group.Compared with the normal group,Improved PAS positive staining area decreased significantly (1 7.61%±3.53%,P<0. 01 ).In normal group and model group,improved hepat-ic glycogen staining showed positive staining of PAS was significantly higher than that of routine hepatic glycogen staining (P<0. 01 ).This suggests that the improved PAS staining of liver glycogen positive stained area more truly reflected the degree of acute liver injury.Conclusion:The improved of Periodic acid-schiff (PAS)staining method is helpful to improve the evaluation quality degree of acute hepatic injury.
Pages: 178-181
Year: 2015 Issue: 1
Journal: Chinese Journal of Bioprocess Engineering
Keyword: Hepatic glycogen; Acute hepatic injury; Periodic acid-schiff (PAS); Improvement;
Abstract: Objective:To study in situ staining of glycogen by modified Periodic acid-schiff (PAS)staining on evaluating acute hepatic injury induced by carbon tetrachloride (CCl4)in rats.Methods:Rats were randomly divided into 2 groups:normal group and model group.Except normal rats,all the other rats in model group were injected subcutaneously 1 00% CCl4 3mL/kg once, then 50% CCl4 2mL/kg two times a week for 4 times.All the rats were sacrificed on the 1 5th day.Liver body ratio of rats was calculated;The serum parameters of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bilirubin (TBil), albumin (Alb)were assayed.Hepatic inflammation was observed with HE staining.Hepatic glycogen was stained by routine PAS staining and improved PAS staining.Results:Compared with the normal group,the activities of ALT and AST and TBil content in-creased significantly (P<0. 05 ).Alb content was significantly decreased compared with the normal group (P<0. 05 ).HE stai-ning showed that serious structural damage,hepatic steatosis and ballooning degeneration were seen in model group.Routine PAS staining showed that PAS positive staining area accounted for 32.38% ±5.50% in normal group.Compared with the normal group,PAS positive staining area decreased significantly (8.60%±3.34 %,P<0. 01 ).Improved PAS staining showed that PAS positive staining area accounted for 75 .50%±9.02 % in normal group.Compared with the normal group,Improved PAS positive staining area decreased significantly (1 7.61%±3.53%,P<0. 01 ).In normal group and model group,improved hepat-ic glycogen staining showed positive staining of PAS was significantly higher than that of routine hepatic glycogen staining (P<0. 01 ).This suggests that the improved PAS staining of liver glycogen positive stained area more truly reflected the degree of acute liver injury.Conclusion:The improved of Periodic acid-schiff (PAS)staining method is helpful to improve the evaluation quality degree of acute hepatic injury.
Related Articles