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Acute Toxicity of Nano-realgar
Author(s): LI Yi-nuo, TIAN Ye, ZHAO Yu, WANG Xiao-bo, XI Rong-gang, ZHANG Zhi-ran, Department of Pharmacy, School of Pharmaceutical Sciences, Liaoning University of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, School of Pharmaceutical Sciences, Harbin Medical University, Pharmacological Base, Hospital 210 of PLA
Pages: 13-
16
Year: 2015
Issue:
1
Journal: Pharmaceutical Journal of Chinese People's Liberation Army
Keyword: nano-realgar; acute toxicity; pathological sections; mice;
Abstract: Objective To explore the acute toxicity of realgar nano-particles in mice in order to facilitate its clinical application. Methods 60 mice were randomly and evenly divided into six groups that received intragastric administration. The control group was given 0. 5% sodium carboxymethyl cellulose while the other groups were given1031,515. 5,257. 8,128. 8,64 mg·kg- 1realgar nanometer suspension. The intragastric volume weight ratio was 25mg·kg- 1. To observe the toxic reaction and death rate of mice,we calculated the median lethal dose( LD50) with Bliss method. The dead mice in each group were anatomized quickly,and their heart,liver,spleen,lung and kidney were taken out for pathological examination. Results The activity of the mice was reduced after administration,without eating or drinking,but with other symptoms,such as shortness of breath and increase of secretion of the eyeball.All mice died in the 1031 mg·kg- 1group,nine in 515. 5 mg·kg- 1group,two in 257. 8 mg·kg- 1group,and one in128. 8 mg·kg- 1group,but norne died in the 64 mg·kg- 1group. Calculated with the Bliss method,the LD50was309. 72 mg·kg- 1,with the 95% confidence limits( Feiller correction) 226. 97- 422. 93 mg·kg- 1; LD5was137. 36mg·kg- 1,LD95 was 698. 35 mg·kg- 1. Autopsy showed diffuse congestion of the lung and liver,swelling and blackening of the spleen while the surviving mice remained normal. Conclusion No obvious acute toxicity symptoms are found in mice administrated with the 64. 4 mg·kg- 1dose of realgar nanoparticles,but when the dose exceeded64. 4 mg·kg- 1,the poisoning of mice will be intensified with the dose.
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