The research about the function of mast cells in proliferation and involution of infantile hemangioma
Author(s): Zhang Jun, Wang Shan, Zhu Jin, Wei Guanghui
Pages: 905-908
Year: 2014 Issue: 12
Journal: Chinese Journal of Pediatric Surgery
Keyword: Hemangioma; Mast cells; Vascular endothelial growth factors; Tryptases;
Abstract: Objective To explore the function of mast cells in proliferation and involution of infantile hemangioma.Methods Paraffin-embedded sections of 22 proliferating,20 involuting and 23 involuted IH lesions were used to investigate the expression of tryptase,VEGF-A and GLUT-1 by immunostaining.The nuclei of all cells were stained by 4',6'-diamidino-2-phenylindole (DAPI).The expression of VEGF and tryptase were observed.The tryptase + mast cells and the percent of VEGF-A + cells in the whole tryptase + mast cells were calculated in all three phases of IH.Results The expression of Glut-1 in all sections of 22 proliferating,20 involuting and 23 involuted IH lesions were all positive.Mast cells,identified by their expression of tryptase,were located in the interstitium of vessels composed by vascular endothelial cells.The average numbers of tryptase + cells in each 9 views of proliferating,involuting and involuted IH were 27.26 ± 4.10,82.20 ± 5.27,44.59 ± 3.07,respectively,which difference is obviously (P＜0.01).100％,(22.00 ± 1.41) ％,(13.00 ± 1.38) ％ ofthese tryptase + cells also expressed VEGF,in proliferating,involuting and involuted IH,respectively,which was difference is also obviously (P＜0.01).Conclusions VEGF-A which was secreted by the most cells plays a vital role in the process of proliferation of IH.
Pages: 905-908
Year: 2014 Issue: 12
Journal: Chinese Journal of Pediatric Surgery
Keyword: Hemangioma; Mast cells; Vascular endothelial growth factors; Tryptases;
Abstract: Objective To explore the function of mast cells in proliferation and involution of infantile hemangioma.Methods Paraffin-embedded sections of 22 proliferating,20 involuting and 23 involuted IH lesions were used to investigate the expression of tryptase,VEGF-A and GLUT-1 by immunostaining.The nuclei of all cells were stained by 4',6'-diamidino-2-phenylindole (DAPI).The expression of VEGF and tryptase were observed.The tryptase + mast cells and the percent of VEGF-A + cells in the whole tryptase + mast cells were calculated in all three phases of IH.Results The expression of Glut-1 in all sections of 22 proliferating,20 involuting and 23 involuted IH lesions were all positive.Mast cells,identified by their expression of tryptase,were located in the interstitium of vessels composed by vascular endothelial cells.The average numbers of tryptase + cells in each 9 views of proliferating,involuting and involuted IH were 27.26 ± 4.10,82.20 ± 5.27,44.59 ± 3.07,respectively,which difference is obviously (P＜0.01).100％,(22.00 ± 1.41) ％,(13.00 ± 1.38) ％ ofthese tryptase + cells also expressed VEGF,in proliferating,involuting and involuted IH,respectively,which was difference is also obviously (P＜0.01).Conclusions VEGF-A which was secreted by the most cells plays a vital role in the process of proliferation of IH.
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