Molecular Cloning,Bioinformatics Analysis of rbcLGene in Bellis perennis
Author(s): XIONG Yong, ZHAO Chun-yan, YANG Qing-song, ZHANG Wei-han, Key Laboratory of Ethnic Medicine Resource Chemistry, State Ethnic Affairs Commission and Ministry of Education, Yunnan University of Nationalities, Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooporatives
Pages: 89-95
Year: 2015 Issue: 4
Journal: Northern Horticulture
Keyword: Bellis perennis; rbcL gene; protein structure prediction; adaptive evolution;
Abstract: Taking Bellis perennis as experimental materials.Bellis perennis rbcL gene was amplified using PCR method and sequenced.Biological softwares DNAstar,PAML was used to bioinformatic and adaptive evolutionary analysis for this gene.The results showed that the full length of cDNA of Bellis perennis rbcLgene was 1 458 bp,it encoded 485 amino acids.Compared to the sequences of this gene in other compositae plants,homology of their nucleotide and amino acid sequences were over 96%.The secondary structure of RbcLprotein had 20α?helices,17β?sheets,33 turns and some coils structures.The adaptive evolutionary analysis using variableωratio sites method indicated that 3amino acid residues(94E,149 Qand 309M)were positively selected.The isolation of rbcLgene will facilitate the further research in Bellis perennis adapting to particular environment.The spatial locations of the positively selected sites inrbcLsubunit played an important role to maintain function of Rubisco.
Pages: 89-95
Year: 2015 Issue: 4
Journal: Northern Horticulture
Keyword: Bellis perennis; rbcL gene; protein structure prediction; adaptive evolution;
Abstract: Taking Bellis perennis as experimental materials.Bellis perennis rbcL gene was amplified using PCR method and sequenced.Biological softwares DNAstar,PAML was used to bioinformatic and adaptive evolutionary analysis for this gene.The results showed that the full length of cDNA of Bellis perennis rbcLgene was 1 458 bp,it encoded 485 amino acids.Compared to the sequences of this gene in other compositae plants,homology of their nucleotide and amino acid sequences were over 96%.The secondary structure of RbcLprotein had 20α?helices,17β?sheets,33 turns and some coils structures.The adaptive evolutionary analysis using variableωratio sites method indicated that 3amino acid residues(94E,149 Qand 309M)were positively selected.The isolation of rbcLgene will facilitate the further research in Bellis perennis adapting to particular environment.The spatial locations of the positively selected sites inrbcLsubunit played an important role to maintain function of Rubisco.
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