Determination of plasma concentration of oxcarbazepine active metabolite in epilepsy patients by HPLC and evaluation of the relationship between concentration and efficacy
Author(s): ZHANG Quan-ying, XU Wen-jun, YU Yun-li, Department of Clinical Pharmacology, The Second Affiliated Hospital of Soochow University
Pages: 106-109
Year: 2015 Issue: 1
Journal: Chinese Journal of Biochemical Pharmaceutics
Keyword: oxcarbazepine; HPLC; MHD; therapeutic drug monitoring;
Abstract: Objective To develop an HPLC method for the determination of 10-hydroxycarbamazepine( MHD) of oxcarbazepine active metabolite in human plasma and evaluate the relationship between concentration and efficacy. Methods Using 6-methoxysalicylic acid as internal standard,MHD was extracted by ethyl acetate. A Symmetry ShiledTMRP18column( 4. 6 × 250 mm,5 μm) was used,with the mobile phase of acetonitrile and water containing 0. 05% formic acid( v / v,23∶77) at the detection wavelength of 214 nm. The flow rate was 1. 0 m L / min and the column temperature was 40℃. Measured concentrations of MHD from 108 blood samples of 78 epileptic patients by HPLC-UV,and all corresponding clinical data were collected from the medical records of the population. The relationship between serum concentration and clinical efficacy was evaluated by assessing curative effect criterion. Results The calibration curve was linear in the range of 0. 4 ~ 40 μg / m L( r = 0. 998) and the detection limit was 0. 4 μg / m L. The RSD of intra-and inter-day precision was both less than 15%. The range of extraction recovery ratio of MHD was 75. 1% ~ 78. 5%. Extraction recovery ratio of internal standard was 86. 5%. The results of therapeutic drug concentration monitoring( TDM) for MHD showed that after taking epilepsy about 2 h,plasma drug concentration reached its peak,there was a large difference between different patients blood drug concentration. Conclusion The method is simple,accurate,sensitive. It is suitable for clinical to study MHD blood concentration monitioring of patients after taking OXC. It is more effective for most patients with epilepsy that the therapeutic drug concentration of MHD is higher than 8 μg / m L.
Pages: 106-109
Year: 2015 Issue: 1
Journal: Chinese Journal of Biochemical Pharmaceutics
Keyword: oxcarbazepine; HPLC; MHD; therapeutic drug monitoring;
Abstract: Objective To develop an HPLC method for the determination of 10-hydroxycarbamazepine( MHD) of oxcarbazepine active metabolite in human plasma and evaluate the relationship between concentration and efficacy. Methods Using 6-methoxysalicylic acid as internal standard,MHD was extracted by ethyl acetate. A Symmetry ShiledTMRP18column( 4. 6 × 250 mm,5 μm) was used,with the mobile phase of acetonitrile and water containing 0. 05% formic acid( v / v,23∶77) at the detection wavelength of 214 nm. The flow rate was 1. 0 m L / min and the column temperature was 40℃. Measured concentrations of MHD from 108 blood samples of 78 epileptic patients by HPLC-UV,and all corresponding clinical data were collected from the medical records of the population. The relationship between serum concentration and clinical efficacy was evaluated by assessing curative effect criterion. Results The calibration curve was linear in the range of 0. 4 ~ 40 μg / m L( r = 0. 998) and the detection limit was 0. 4 μg / m L. The RSD of intra-and inter-day precision was both less than 15%. The range of extraction recovery ratio of MHD was 75. 1% ~ 78. 5%. Extraction recovery ratio of internal standard was 86. 5%. The results of therapeutic drug concentration monitoring( TDM) for MHD showed that after taking epilepsy about 2 h,plasma drug concentration reached its peak,there was a large difference between different patients blood drug concentration. Conclusion The method is simple,accurate,sensitive. It is suitable for clinical to study MHD blood concentration monitioring of patients after taking OXC. It is more effective for most patients with epilepsy that the therapeutic drug concentration of MHD is higher than 8 μg / m L.
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