Establishment of Aminoglycoside resistance gene of foodborne MRSA detection method by Multiple- PCR
Author(s): ZHAN Xiao- wei, ZHENG Qiu-yue, FU Jun-fan, XU Jun-yi, CHEN Lu, DONG Shu-jun, Shenyang Agricultural University, College of Plant Protection, Liaoning Entry- Exit Inspection and Quarantine Bureau, Shenyang Municipal Drainage Management, Dalian NO.24 High School
Pages: 303-308
Year: 2015 Issue: 1
Journal: Science and Technology of Food Industry
Keyword: MRSA; aminoglycoside; resistant gene;
Abstract: Objective: To investigate the prevalence of methicillin- resistant Staphylococcus aureus MRSA and resistance to Aminoglycoside gentamicin,kanamycin in foodborne S.aureus,and establish a rapid quadruple- PCR method for detection and identification antibiotic resistance of S.aureus.Methods: Genomic DNA was extracted as template for PCR from S.aureus recoveried and cultured in enrichment medium.According to nuc for Genus,mec A for MRSA,aac A- aph D and aph( 3’)- Ⅲa for Gentamicin and kanamycin,quadruple- RCR detection method was developed,its sensitivity and specificity were evaluated; a total of 72 strains of S.aureus from food and patients with food poisoning were detected by quadruple RCR method. Results: Quadruple- RCR detection method was established.72 strains of S.aureus all had nuc,the coincidence rate of mec A and MRSA was 100%,the coincidence rate of aac A- aph D,aph( 3’)- Ⅲa and gentamicin,kanamycin was 95.12%.Conclusion: this established quadruple- RCR method was specific,sensitive. MRSA could be tested rapidly,and there was high accurate rate for detection of gentamicin,kanamycin resistant strains of S.aureus.
Pages: 303-308
Year: 2015 Issue: 1
Journal: Science and Technology of Food Industry
Keyword: MRSA; aminoglycoside; resistant gene;
Abstract: Objective: To investigate the prevalence of methicillin- resistant Staphylococcus aureus MRSA and resistance to Aminoglycoside gentamicin,kanamycin in foodborne S.aureus,and establish a rapid quadruple- PCR method for detection and identification antibiotic resistance of S.aureus.Methods: Genomic DNA was extracted as template for PCR from S.aureus recoveried and cultured in enrichment medium.According to nuc for Genus,mec A for MRSA,aac A- aph D and aph( 3’)- Ⅲa for Gentamicin and kanamycin,quadruple- RCR detection method was developed,its sensitivity and specificity were evaluated; a total of 72 strains of S.aureus from food and patients with food poisoning were detected by quadruple RCR method. Results: Quadruple- RCR detection method was established.72 strains of S.aureus all had nuc,the coincidence rate of mec A and MRSA was 100%,the coincidence rate of aac A- aph D,aph( 3’)- Ⅲa and gentamicin,kanamycin was 95.12%.Conclusion: this established quadruple- RCR method was specific,sensitive. MRSA could be tested rapidly,and there was high accurate rate for detection of gentamicin,kanamycin resistant strains of S.aureus.
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