Enantiomer Separation of Mexiletine Hydrochloride with Carboxymethyl-β-Cyclodextrin as Chiral Mobile Phase Addtive
Author(s): Yu hao, Su Liqiang, Zhai Minghui, Zhang Weibing
Pages: 8-10
Year: 2014 Issue: 12
Journal: Chemical Industry Times
Keyword: CM-β-CD; chiral mobile phase additive; HPLC; mexiletine hydrochloride enantiomer;
Abstract: Separation of Mexiletine hydrochloride enantiomers by HPLC was established on C18 column using the carboxymethyl-β-cyclodextrin ( CM-β-CD in short) as chiral mobile phase additive. The influences of concentration of chiral mobile phase additive, the organic modifier, the pH value and the flow rate on the enantiomers separation were investigated systematically. The better separation was achieved when the mobile phase consist of methanol-acetoni-trile-water 15∶70∶15(V/V/V), pH=4. 28( adjusted by triethylamine-glacial acetic acid ), containing 0. 5 g· L-1 CM-β-CD, at the flow rate of 0. 3 mL·min-1 . The detection wavelength was set at 212 nm.
Pages: 8-10
Year: 2014 Issue: 12
Journal: Chemical Industry Times
Keyword: CM-β-CD; chiral mobile phase additive; HPLC; mexiletine hydrochloride enantiomer;
Abstract: Separation of Mexiletine hydrochloride enantiomers by HPLC was established on C18 column using the carboxymethyl-β-cyclodextrin ( CM-β-CD in short) as chiral mobile phase additive. The influences of concentration of chiral mobile phase additive, the organic modifier, the pH value and the flow rate on the enantiomers separation were investigated systematically. The better separation was achieved when the mobile phase consist of methanol-acetoni-trile-water 15∶70∶15(V/V/V), pH=4. 28( adjusted by triethylamine-glacial acetic acid ), containing 0. 5 g· L-1 CM-β-CD, at the flow rate of 0. 3 mL·min-1 . The detection wavelength was set at 212 nm.
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