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Rapid detection method preliminary establishment of infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification
Pages: 12-17
Year: Issue:  12
Journal: Liaoning Journal of Animal Husbandry & Veterinary Medicine

Keyword:  IBDVLoop-mediated isothermal amplificationDiagnosis method;
Abstract: The objective of this study is to develop a rapid and sensitive reverse transcription loop- mediated isothermal amplification(RT- LAMP) method for the detection of infectious bursal disease virus(IBDV). Six primers were designed to amplify the VP1 gene of IBDV using Primer Explorer V4. The optimal reaction condition of the current RT-LAMP for IBDV was 65 ℃ for 60 mins. It was capable of detecting IBDV from clinical samples and differentiating IBDV from other avain viruses, and does not require an additional expensive equipment. The minimum detection limit of the RT-LAMP assay was 10- 6, making this assay approximately 100-fold higher than that of one-step RTPCR. The assay were evaluated by comparison with RT-PCR, by 93.8% consistence. The specificity and simplicity of the assay could make it a useful method for the detection of IBDV infection.
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