Author(s): YANG Zuo, GAO Lin, ZUO Yonghui, WANG Xiaoying, LIAO Zuo, HUANG Zuo, ZHANG Zuo, CHEN Qirong
Pages: 1115-1119
Year: 2014 Issue:  12
Journal: Chinese Journal of Endocrinology and Metabolism
Keyword:  1; 25-dihydroxyvitamin D3; Diabetes mellitus; experimental; Peroxisome proliferator-activated receptor α; Hepatocyte neclear factor-4α; Sterol regulatory element binding proteins-1C; Apolipoprotein A5; Triglyceride;
Abstract: Objective Activity of vitamin D is correlated with type 2 diabetes mellitus and hyperlipidemia,but the concrete relationship of 1,25-dihydroxyvitamin D3 [1,25 (OH) 2 D3] and triglyceride is unknown.This study was to observe the gene and protein expression of peroxisome proliferator-activated receptor α(PPAR-α),hepatocyte neclear factor-4α (HNF-4α),sterol regulatory element binding proteins-1C (SREBP-1 C),apolipoprotein (apoA5) during 1,25 (OH)2D3 regulation of triglyceride in liver of diabetes rat,as well as to investigate the mechanism of 1,25 (OH) 2D3 in influencing triglyceride in liver.Methods Eighty male Wistar Rats were divided into 4 groups:20 rats with diabetes mellitus(DM group),20 rats of 1,25 (OH)2D3 treated group (V itD group),20 rats with PPAR-α inhibition group,and 20 rats of normal control group.15 from each group were taken in random by random number table to detect biochemical indicators and to detect the gene and protein expressions of PPAR-α,HNF-4α,SREBP-1 C,apoA5 in liver,using realtime-fluorescent quantitation reverse transcription,polymerase chain reaction and Western blot.The level of triglyceride in liver was also detected.Results (1) The gene and protein expression of PPAR-α,HNF-4α,apoA5 in liver of diabetic rat was lower than that in normal control group (P ＜ 0.05).The expression of SREBP-1C and the level of triglyceride in liver of diabetic rat were higher than those of normal control group (P＜0.05).(2)The expression of PPAR-α,HNF-4α,apoA5 in liver of VitD group were higher than those of DM group(P＜0.05).The gene and protein expression of SREBP-1C and the level of triglyceride in VitD group were lower than those in DM group(P＜0.05).(3) The expression of PPAR-α,HNF-4α,SREBP-1 C,apoA5,and the level of triglyceride showed no significant difference between VitD group and normal control group(P＞0.05).(4) The expression of PPAR-α,HNF-4α,and apoA5 in liver of PPAR-α inhibition group was lower than that in normal control group(P＜0.05).The expression of SREBP-1C and the level of triglyceride in liver of PPAR-α inhibition group were higher than those in normal control group(P＜0.05).(5) The expression of PPAR-α in liver of PPAR-α inhibition group was lower than that in diabetic rat (P＜0.05).The expression of HNF-4α,apoA5,SREBP-1 C,and the level of triglyceride showed no significant difference between PPAR-α inhibition group and diabetic rat(P＞0.05).(6) The expression of PPAR-α,HNF-4α,apoA5 in liver of PPAR-α inhibition group was lower than that in VitD group(P＜ 0.05).The expression of SREBP-1C and the level of triglyceride in liver of PPAR-α inhibition group were higher than those in VitD group (P ＜ 0.05).Conclusions 1,25 (OH) 2D3 may lower the level of serum triglyceride by upregulating the expression of PPAR-α,HNF-4α,and apoA5 and inhibiting the expression of SREBP-1C in liver.