Urine metabonomic study on long-term use of total ginsenosides in rats
Author(s): XIE Xie, CHEN Shao-qiu, LV Ying-fang, WANG Xiao-yan, JIA Wei, Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Anhui Agriculture University
Pages: 4675-4679
Year: 2014 Issue: 23
Journal: China Journal of Chinese Materia Medica
Keyword: ginsenoside; urine; metabolomics;
Abstract: Due to its effect of systems regulation and promotion on body,Ginseng is always referred to be long-term used as a dietary supplement. But it was still unclear about its target of the tonic effects and also the side-effects long-term use may bring. Urine metabolomic method is suitable for long-term studies of pharmaco-dynamics,pharmacology and toxicology of traditional Chinese medicine because of its characteristics of non-invasive and monitoring the whole-body metabolism. This study was designed to detect the dynamic variation of rat urine metabolome along with a long-term administration of total ginsenosides using GC-TOF based metabolomic technology. Our result showed that either short-term or chronic administration of ginsenosides did not impact the rat urine metabolome significantly( as the PCA subgroup was not successful). By comparison,the short-term( 1-3 w) dose of ginsenosides had the biggest metabolic influence including TCA cycle,catecholamines and neurotransmitter amino acids. Medium-term( 6-10 w) dose had a gradually lower effect and long-term( 27 w) dose almost had no effect. Our study indicates that both short and long term administration of ginsenosides showed almost no obvious side-effect on the experimental animals.
Pages: 4675-4679
Year: 2014 Issue: 23
Journal: China Journal of Chinese Materia Medica
Keyword: ginsenoside; urine; metabolomics;
Abstract: Due to its effect of systems regulation and promotion on body,Ginseng is always referred to be long-term used as a dietary supplement. But it was still unclear about its target of the tonic effects and also the side-effects long-term use may bring. Urine metabolomic method is suitable for long-term studies of pharmaco-dynamics,pharmacology and toxicology of traditional Chinese medicine because of its characteristics of non-invasive and monitoring the whole-body metabolism. This study was designed to detect the dynamic variation of rat urine metabolome along with a long-term administration of total ginsenosides using GC-TOF based metabolomic technology. Our result showed that either short-term or chronic administration of ginsenosides did not impact the rat urine metabolome significantly( as the PCA subgroup was not successful). By comparison,the short-term( 1-3 w) dose of ginsenosides had the biggest metabolic influence including TCA cycle,catecholamines and neurotransmitter amino acids. Medium-term( 6-10 w) dose had a gradually lower effect and long-term( 27 w) dose almost had no effect. Our study indicates that both short and long term administration of ginsenosides showed almost no obvious side-effect on the experimental animals.
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