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Species identification of partial sandflies from China with DNA barcoding
Author(s): ZHOU Zheng-bin, ZHANG Yi, LV Shan, SHI Wen-qi, JIN Chang-fa, ZHU Huai-min
Pages: 1209-
1213
Year: 2014
Issue:
12
Journal: Chinese Journal of Zoonoses
Keyword: sandflies; DNA barcoding;
Abstract: ABSTRACT:To explore the feasibility of DNA barcoding in the identification of sandflies ,the mitochondrial cytochrome c oxidase subunit I (COI) genes of 9 dominant species in China from three genera in the Phlebotominae were studied .The in‐traspecies and interspecies genetic distances were calculated using the Kimura‐2‐parameter model ,and the phylogenetic trees were reconstructed by neighbor‐joining (NJ) method .Results showed that all of sandflies species were successfully distinguish‐ed by the phylogenetic trees .The average intraspecific genetic distance (0 .8% ) was much less than the average interspecific ge‐netic distance (11 .2% ) .The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes ,which are potentially well utilized in sandflies species identification .
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