Author(s): XIE Dong-mei, CHEN Guo-ming, HE De, LI Cui-xin
Pages: 378-384
Year: 2014 Issue:  4
Journal: Guangxi Forestry Science
Keyword:  Pinus armandi; classical CTAB; simple CTAB; modified CTAB; Zigenhagen method;
Abstract: Using leaves of Pinus armandi as experimental materials, genomic DNA was extracted using four methods, which were the classical CTAB method, simple CTAB method, modified CTAB method and Zigenhagen method and were analyzed by agarose gel electrophoresis, ultraviolet spectrophotometer, standard deviation and standard error of arithmetic mean, Chi-square test and SPSS. The result of the Chi-square test showed that the differences of the data were derived from the method, not the accidental error. According to the analysis of ( OD260 - OD320 ) / ( OD280 - OD320 ) , the standard deviation and standard error of modified CTAB method was the lowest. The classical CTAB method was the second. The simple CTAB method was the third. It implied that stability and the data precision were the best by modi-fied CTAB method. The results of the ultraviolet spectrophotometer and SPSS showed that the ratio (OD260 - OD320 ) / ( OD280 -OD320 ) of classical CTAB method was greater than 1. 9, which might have RNA pollution. The ratio ( OD260 -OD320 ) / ( OD280 -OD320 ) of simple CTAB method and Zigen-hagen method were less than 1. 80 , which might have little protein pollution. The ratio ( OD260 -OD320 ) / ( OD280 -OD320 ) of modified CTAB method was 1. 855 closed to the theory value (1. 80) with the lowest standard deviation and standard error, which indicated that stability and precision of data were the best and the DNA purity was the best.