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xiang gen cao wai zhi ti de xuan ze ji zhi zhu zai sheng shi yan
Author(s): 
Pages: 7-9
Year: Issue:  6
Journal: Yunnan Agricultural Science and Technology

Keyword:  香根草外植体愈伤组织诱导植株再生;
Abstract: 为建立香根草组织培养及植株再生体系,分别选取生长健壮无病虫害的植株茎杆、根、叶和幼嫩芽不同部位进行外植体选择及愈伤组织诱导,以及香根草外植体消毒方法和激素处理及培养基对丛生芽诱导的影响试验.结果表明,茎杆、根、叶作为外植体,诱导不出愈伤组织.幼嫩芽是最佳香根草外植体的选择.0.1%升汞对各部位处理效果成功率在65%以上,幼嫩芽消毒浸泡8 min成功率达88%.在MS+6-BA 1.5 mg/L+NAA 0.1 mg/L的培养基上培养25 d幼芽愈伤组织诱导率为100%.在MS+6-BA 0.8 mg/L+NAA 0.2 mg/L培养基上培养20 d诱导丛生芽效果最好,增殖系数为6.4.生根培养基选用1/2 MS+NAA 0.3 mg/L+IAA 0.1 mg/L为最佳,生根率达90%以上.
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