Determination of Hyperoside in the Qianbai Biyan Pian by HPLC
Author(s): SU Jian
Pages: 80-81,91
Year: 2014 Issue: 6
Journal: Journal of Kunming Teachers College
Keyword: senecionis scandentis hebra; qianbai biyan pian; hyperoside; HPLC;
Abstract: Determining Hyperoside from ten batches of qianbaibiyan tablets with HPLC external standard method,the chromatography condition was eighteen alkyl silane bonded silica as filler,V(acetonitrile)∶V(0.2% acetic acid solution)=16∶84 as the mobile phase;the column temperature was 30℃;the flow rate was 1 mL/min;the detection wavelength was 360 nm.Theoretical plate column under this condition number(n)is greater than 10 000;the degree of separation of the target component chromatographic peak(R)greater than 1.5;the tailing factor(T)greater than 0.95 and less than 1.05;require quantitative detection of chromatographic conditions were able to achieve the“Pharmacopoeia of the people′s Republic of China”in 2010 edition of high performance liquid chromatography a the quantita-tive determination and meet the single active ingredient in the compound preparation.The determination results show that Hyperoside in each tablet of the ten batches in qianbaibiyan tablets averaged 0.848 6 mg.The average recovery was 96.90%,RSD was 0.41%(n=9).
Pages: 80-81,91
Year: 2014 Issue: 6
Journal: Journal of Kunming Teachers College
Keyword: senecionis scandentis hebra; qianbai biyan pian; hyperoside; HPLC;
Abstract: Determining Hyperoside from ten batches of qianbaibiyan tablets with HPLC external standard method,the chromatography condition was eighteen alkyl silane bonded silica as filler,V(acetonitrile)∶V(0.2% acetic acid solution)=16∶84 as the mobile phase;the column temperature was 30℃;the flow rate was 1 mL/min;the detection wavelength was 360 nm.Theoretical plate column under this condition number(n)is greater than 10 000;the degree of separation of the target component chromatographic peak(R)greater than 1.5;the tailing factor(T)greater than 0.95 and less than 1.05;require quantitative detection of chromatographic conditions were able to achieve the“Pharmacopoeia of the people′s Republic of China”in 2010 edition of high performance liquid chromatography a the quantita-tive determination and meet the single active ingredient in the compound preparation.The determination results show that Hyperoside in each tablet of the ten batches in qianbaibiyan tablets averaged 0.848 6 mg.The average recovery was 96.90%,RSD was 0.41%(n=9).
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