Author(s): TANG Li-juan, WU Hao, YU Hong-li, Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing
Pages: 3583-3586
Year: 2014 Issue:  11
Journal: China Journal of Traditional Chinese Medicine and Pharmacy
Keyword:  HPLC; Arecae Pericarpium; Arecoline; Arecaidine; Guvacoline; Guvacine;
Abstract: Objective: To determine the content of arecoline, arecaidine, guvacoline and guvacine in Arecae Pericarpium, a simple and rapid HPLC method has been developed. Methods: The separations were carried out at 30℃ on a cation-exchange column(SCX, particle size 5μm, 250mm×4.6mm), eluted with the mobile phase consisted of acetonitril-0.1% phosphoric acid solution(adjusted to pH 3.3 with ammonium hydroxide). The low-rate was 1.0mL/min and the eluent was monitored at 215 nm. Results: Arecoline hydrobromide, arecaidine hydrochloride, guvacoline hydrobromide and guvacine hydrochloride were well separated by this method, and showed a good linearity in the given ranges respectively. The average recoveries of the four alkaloids were 96.9%, 103.0%, 102.8%, 102.3%, RSD of them were 1.82%, 1.45%, 1.75% and 1.12% respectively. Conclusion: The present method is accurate, handy and highly repeatable, can be used to determine the contents of the 4 major alkaloids in Arecae Pericarpium, which provides a scientific basis for the multi-index evaluation and quality control system of Arecae Pericarpium and its preparation.