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Genetic relationships among fifteen species of Kobresia Willd. (Cyperaceae, tribe Cariceae) based on RAPD markers
Author(s): 
Pages: 38-43
Year: Issue:  5
Journal: JOURNAL OF LANZHOU UNIVERSITY(NATURAL SCIENCES)

Keyword:  KobresiaGenetic relationshipRAPD marker;
Abstract: Plants of the genus Kobresia, are dominant alpine sedges of high ecological and economic importance. Phylogenetic relationships of Kobresia species have not been well resolved due to limitations in sample availability. Also, previous studies focused on morphological traits, which can be variable in changing habitats and environmental conditions. Here we examine 15 Kobresia species to estimate the genetic relationships in the genus and to reexamine previous phylogenetic efforts. Twenty-one RAPD primers were chosen to examine 47 accessions of the 15 species. The results indicate that the species cluster into three main groups. Previously the genus was divided into section Kobresia, section Elyna,and section Hemicarex, and these classifications were based upon inflorescence structure. Our molecular results generally correspond with section Kobresia and Hemicarex. However the molecular phylogeny does not match with section Elyna, indicating that Elyna is not a natural classification. Additionally,although K. maquensis has been previously classified as an independent species, our results suggest that it should be considered a synonym of K. setschwanensis. These results also show that RAPD markers are effective in constructing phylogenetic relationships among Kobresia species.
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