Analysis on Polymorphism of CALCA Gene in Zaosheng Cattle and Zaosheng Hybrids Cattle and Their Association with Carcass Trait
Author(s): ZHANG Cai-xia, ZHAO Sheng-guo, LI Tian-ke, LEI Zhao-min
Pages: 114-118
Year: 2014 Issue: 5
Journal: Acta Agriculturae Boreali-Sinica
Keyword: Beef cattle; CALCA gene; Analysis of population genetic variations; Carcass traits;
Abstract: The aim was to analyze association of CALCA gene polymorphisms with some economic traits in Zaosheng cattle and Zaosheng crossbred , and to evaluate it as a candidate molecular marker associated with beef quality.PCR-SSCP method was used to detect the genetic variation in CALCA gene in 362 cattle from 5 cattle groups ,including Zaosheng in Qingyang Prefecture , Zaosheng cattle in Pingliang Prefecture , Nanza ( South Devon cattle ×Qingyang groups),Qinza (Qinchuan cattle ×Pingliang groups),Xiza (Simmental ×Pingliang groups).The association between SNP locus and some carcass traits of Pingliang groups were analyzed .Two genotypes AA and AB were detected at exon 4 in CALCA gene .Analysis of association of polymorphism with carcass traits at mutation locus showed that there were significant effects on WBSF ,Ribeye area ,Hot carcass weight and Cooking loss ( P<0 .05 ) and there was no effect on other traits(P>0.05).In conclusion,SNP of CALCA gene could be a genetic mark for carcass trait.
Pages: 114-118
Year: 2014 Issue: 5
Journal: Acta Agriculturae Boreali-Sinica
Keyword: Beef cattle; CALCA gene; Analysis of population genetic variations; Carcass traits;
Abstract: The aim was to analyze association of CALCA gene polymorphisms with some economic traits in Zaosheng cattle and Zaosheng crossbred , and to evaluate it as a candidate molecular marker associated with beef quality.PCR-SSCP method was used to detect the genetic variation in CALCA gene in 362 cattle from 5 cattle groups ,including Zaosheng in Qingyang Prefecture , Zaosheng cattle in Pingliang Prefecture , Nanza ( South Devon cattle ×Qingyang groups),Qinza (Qinchuan cattle ×Pingliang groups),Xiza (Simmental ×Pingliang groups).The association between SNP locus and some carcass traits of Pingliang groups were analyzed .Two genotypes AA and AB were detected at exon 4 in CALCA gene .Analysis of association of polymorphism with carcass traits at mutation locus showed that there were significant effects on WBSF ,Ribeye area ,Hot carcass weight and Cooking loss ( P<0 .05 ) and there was no effect on other traits(P>0.05).In conclusion,SNP of CALCA gene could be a genetic mark for carcass trait.
Related Articles