Author(s): DENG Guangcun, WANG Yujiong, XU Chongbo, ZENG Zuo
Pages: 76-78
Year: 2002 Issue:  1
Journal: JOURNAL OF NINGXIA UNIVERSITY(NATURAL SCIENCE EDITION)
Keyword:  大肠杆菌; K99基因; 亚克隆; 核苷酸序列;
Abstract: 利用PCR技术,从含有大肠杆菌菌毛抗原K99基因的质粒pX5K中扩增出0.4kb的K99菌毛抗原基因,然后将其亚克隆到pGEM--easy载体上,并转化至受体菌JM109中,采用碱性裂解法提取质粒DNA后,经Ncol和EcoR I双酶切分析和核苷酸序列分析,证明插入的K99基因片段具有正确的核苷酸序列.